Clone:
REA754
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
p150, integrin αX, ITGAX, CR4

Extended validation for CD11c Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA754
N418++
HL3++
Cells were incubated with an excess of purified unconjugated CD11c (REA754) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD11c. Splenocytes from C57BL/6 mice were stained with CD11c antibodies and plotted against the side scatter. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Splenocytes from C57BL/6 mice were stained with CD11c antibodies and plotted against the side scatter. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Splenocytes from C57BL/6 mice were stained with CD11c antibodies and plotted against the side scatter. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Splenocytes from C57BL/6 mice were stained with CD11c antibodies and plotted against the side scatter. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Splenocytes from C57BL/6 mice were stained with CD11c antibodies and plotted against the side scatter. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD11c. Splenocytes from C57BL/6 mice were stained with CD11c antibodies and plotted against the side scatter. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11c (REA754). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11c (REA754). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD11c (REA754). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD11c Antibody, anti-mouse, REAfinity™

Overview

Clone REA754 recognizes the mouse CD11c antigen, also known as integrin αX or Itgax. CD11c is present in dendritic cells in lymphoid organs and blood, in Langerhans cells in the epidermis, in dendritic cell progenitors in the bone marrow, and in
in vitro
–generated bone marrow–derived dendritic cells. In spleen and lymph node, CD11c is found at high levels on conventional CD11c
+
CD45R
mPDCA-1
dendritic cells, and at moderate levels on CD11c
+
CD45R
+
mPDCA-1
+
plasmacytoid dendritic cells. CD11c is reported to be weakly expressed on NK cells, B cells, and T cell subsets. About 1–3% of splenocytes, 2% of bone marrow cells, as well as <1% of lymph node cells and thymocytes express CD11c. CD11c is a receptor for fibrinogen. It mediates cell-cell interaction during inflammatory responses and is involved in monocyte adhesion and chemotaxis.
Additional information: Clone REA754 displays negligible binding to Fc receptors.

Alternative names

p150, integrin αX, ITGAX, CR4

Detailed product information

Technical specifications

CloneREA754
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD11c
Alternative names of antigenp150, integrin αX, ITGAX, CR4
Molecular mass of antigen [kDa]127
Distribution of antigenT cells, NK cells, B cells, dendritic cells, granulocytes, macrophages, monocytes, lymphocytes
Entrez Gene ID16411
RRIDAB_2654706, AB_2654707, AB_2654708, AB_2654709, AB_2654710, AB_2654711, AB_2654712, AB_2654713, AB_2654714, AB_2654715, AB_2654716, AB_2654717, AB_2654718, AB_2654719, AB_2654705

Resources for CD11c Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD11c Antibody, anti-mouse, REAfinity™

Publications

  1. Springer, T. A. (1994) Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76(2): 301-314
  2. López-Rodríguez, C. et al. (1996) AP-1 regulates the basal and developmentally induced transcription of the CD11c leukocyte integrin gene. J. Immunol. 156(10): 3780-3787

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