Clone:
REA325
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
ITGAM, ITGAX, Leukocyte adhesion glycoprotein p150,95 α chain (Leukocyte adhesion receptor p150,95), RGD1561123, CR-3 α chain, Cell surface glycoprotein MAC-1 subunit α, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b

Extended validation for CD11b/c Antibody, anti-rat, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA325
OX-42++
WT.5 (only CD11b)-
Cells were incubated with an excess of purified unconjugated CD11b/c (REA325) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD11b/c. Splenocytes from Wistar rat were stained with CD11b/c antibodies and with a suitable counterstaining. As a control, CD11b/c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b/c. Splenocytes from Wistar rat were stained with CD11b/c antibodies and with a suitable counterstaining. As a control, CD11b/c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b/c. Splenocytes from Wistar rat were stained with CD11b/c antibodies and with a suitable counterstaining. As a control, CD11b/c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b/c. Splenocytes from Wistar rat were stained with CD11b/c antibodies and with a suitable counterstaining. As a control, CD11b/c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD11b/c. Splenocytes from Wistar rat were stained with CD11b/c antibodies and with a suitable counterstaining. As a control, CD11b/c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11b/c (REA325). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11b/c (REA325). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD11b/c (REA325). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD11b/c Antibody, anti-rat, REAfinity™

Overview

Clone REA325 recognizes the rat CD11b and c antigen, which are both single-pass type I membrane glycoproteins also known as integrin α-M (CD11b) and integrin α-X (CD11c). CD11c and CD11b are expressed on monocytes, granulocytes, macrophages, dendritic cells, NK cells, and a subset of lymphocytes.
Additional information: Clone REA325 displays negligible binding to Fc receptors.

Alternative names

ITGAM, ITGAX, Leukocyte adhesion glycoprotein p150,95 α chain (Leukocyte adhesion receptor p150,95), RGD1561123, CR-3 α chain, Cell surface glycoprotein MAC-1 subunit α, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b

Detailed product information

Technical specifications

CloneREA325
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesrat
AntigenCD11b/c
Alternative names of antigenITGAM, ITGAX, Leukocyte adhesion glycoprotein p150,95 α chain (Leukocyte adhesion receptor p150,95), RGD1561123, CR-3 α chain, Cell surface glycoprotein MAC-1 subunit α, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b
Molecular mass of antigen [kDa]128
Distribution of antigenmonocytes, macrophages, granulocytes, dendritic cells, NK cells, lymphocytes
Entrez Gene ID25021, 499271
RRIDAB_2752041, AB_2752218, AB_2752215, AB_2811490, AB_2857720, AB_2876964, AB_2654680, AB_2654681, AB_2654692, AB_2752052

Resources for CD11b/c Antibody, anti-rat, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD11b/c Antibody, anti-rat, REAfinity™

Publications

  1. Hubert, F. X. et al. (2004)
    Rat plasmacytoid dendritic cells are an abundant subset of MHC Class II
    +
    CD4
    +
    CD11b
    OX62
    and type I IFN-producing cells that exhibit selective expression of Toll-like receptors 7 and 9 and strong responsiveness to CpG.
    J. Immunol. 172(12): 7485-7494
  2. Kriegel, M. A. et al. (2012)
    Pancreatic islet expression of chemokine CCL2 suppresses autoimmune diabetes via tolerogenic CD11c
    +
    CD11b
    +
    dendritic cells.
    Proc. Natl. Acad. Sci. U.S.A. 109(9): 3457-3462
  3. Milling, S. et al. (2010) Subsets of migrating intestinal dendritic cells. Immunol. Rev. 234(1): 259-267

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