Clone:
REAL113
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
integrin αM, Mac-1 α, Integrin alpha-M, CR3A, ITGAM, MAC1A, MO1A, SLEB6

Extended validation for CD11b Antibody, anti-mouse,
REAlease
®

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REAL113
M1/70++
M1/70.15.11.5++
5C6+
REA592++
Cells were incubated with an excess of purified unconjugated CD11b (REAL113) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD11b Antibody, anti-mouse,
REAlease
®

Overview

Clone REAL113 is an antibody fragment derived from the full CD11b antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL113 recognizes the human and mouse CD11b antigen (Mac-1 α; integrin αM chain), which is part of the CD11b/CD18 heterodimer (Mac-1 α, Mβ2 integrin), also known as the C3 complement receptor. It functions as a receptor for complement (C3bi), fibrinogen, or clotting factor X. In humans, CD11b is strongly expressed on myeloid cells and weakly expressed on natural killer (NK) cells and some activated lymphocytes as well as on microglia in the brain. In mice, the CD11b antigen is expressed on monocytes/macrophages and microglia. To a lower extent it is expressed on granulocytes, NK cells, CD5
+
B-1 cells, and subsets of dendritic cells. Clone REAL113 recognizes also the non-human primate CD11b antigen.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

integrin αM, Mac-1 α, Integrin alpha-M, CR3A, ITGAM, MAC1A, MO1A, SLEB6

Detailed product information

Technical specifications

CloneREAL113
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Speciesmouse
Cross-reactivitynon-human primate
AntigenCD11b
Alternative names of antigenintegrin αM, Mac-1 α, Integrin alpha-M, CR3A, ITGAM, MAC1A, MO1A, SLEB6
Distribution of antigenmyeloid cells, NK cells, microglia, lymphocytes, brain
RRIDAB_2783918, AB_2811689

Resources for CD11b Antibody, anti-mouse,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD11b Antibody, anti-mouse,
REAlease
®

Publications

  1. Pahl, H. L. et al. (1992) Characterization of the myeloid-specific CD11b promoter. Blood 79(4): 865-870
  2. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice. J. Neurosci. 30(29): 9651-9658
  3. Liu, T. et al. (2014)
    Gr-1
    +
    CD11b
    +
    cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.
    Sci Rep 4: 4833

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