Clone:
REA592
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
integrin αM, Mac-1 α, Integrin alpha-M, CR3A

Extended validation for CD11b Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA592
M1/70++
M1/70.15.11.5++
5C6+
REAL113++
Cells were incubated with an excess of purified unconjugated CD11b (REA592) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD11b. C57BL/6 cells were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. C57BL/6 cells were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. C57BL/6 cells were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. C57BL/6 cells were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. C57BL/6 cells were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD11b. C57BL/6 cells were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11b (REA592). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11b (REA592). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD11b (REA592). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD11b Antibody, anti-mouse, REAfinity™

Overview

Clone REA592 recognizes the mouse CD11b antigen (Mac-1 α; integrin αM chain), which is part of the CD11b/CD18 heterodimer (Mac-1 α, Mβ2 integrin), also known as the C3 complement receptor. It functions as a receptor for complement (C3bi), fibrinogen, or clotting factor X. CD11b is expressed on monocytes, macrophages, and microglia. To a lower extent it is expressed on granulocytes, NK cells, CD5
+
B-1 cells, and subsets of dendritic cells. Clone REA592 recognizes also the non-human primate and human CD11b antigen.
Additional information: Clone REA592 displays negligible binding to Fc receptors.

Alternative names

integrin αM, Mac-1 α, Integrin alpha-M, CR3A

Detailed product information

Technical specifications

CloneREA592
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse, non-human primate, human
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD11b
Alternative names of antigenintegrin αM, Mac-1 α, Integrin alpha-M, CR3A
Molecular mass of antigen [kDa]126(same molecular weight for human and mouse/rat)
Distribution of antigenNK cells, microglia, lymphocytes, monocytes, macrophages
Entrez Gene ID3684, 16409
RRIDAB_2726049, AB_2751172, AB_2726324, AB_2726327, AB_2726328, AB_2726326, AB_2726050, AB_2751173, AB_2751107, AB_2819369, AB_2751174, AB_2733498, AB_2733499, AB_2726325

Resources for CD11b Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD11b Antibody, anti-mouse, REAfinity™

Publications

  1. Pahl, H. L. et al. (1992) Characterization of the myeloid-specific CD11b promoter. Blood 79(4): 865-870
  2. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice. J. Neurosci. 30(29): 9651-9658
  3. Liu, T. et al. (2014)
    Gr-1
    +
    CD11b
    +
    cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.
    Sci Rep 4: 4833

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