Clone:
M1/70.15.11.5
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGAM, CR3A, Mac-1 α, MAC1A, MO1A, SLEB6, integrin αM

Extended validation for CD11b Antibody, anti-human/mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with M1/70.15.11.5
M1/70+
REA592++
ICRF44-
CBRM1/5-
REA713-
Cells were incubated with an excess of purified unconjugated CD11b (M1/70.15.11.5) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD11b. Human peripheral blood cells (PBMCs) after erythrocyte lysis were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. Human peripheral blood cells (PBMCs) after erythrocyte lysis were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. Human peripheral blood cells (PBMCs) after erythrocyte lysis were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. Human peripheral blood cells (PBMCs) after erythrocyte lysis were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. Human peripheral blood cells (PBMCs) after erythrocyte lysis were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11b. Human peripheral blood cells (PBMCs) after erythrocyte lysis were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD11b. Human peripheral blood cells (PBMCs) after erythrocyte lysis were stained with CD11b antibodies and plotted against the side scatter. As a control, CD11b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11b (M1/70.15.11.5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11b (M1/70.15.11.5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD11b (M1/70.15.11.5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD11b Antibody, anti-human/mouse

Overview

CD11b, also known as Mac-1 α or integrin αM chain, is part of the CD11b/CD18 heterodimer (Mac-1 α, Mβ2 integrin), also known as the C3 complement receptor. It functions as a receptor for complement (C3bi), fibrinogen, or clotting factor X. In humans, CD11b is strongly expressed on myeloid cells and weakly expressed on NK cells and some activated lymphocytes as well as on microglia in the brain. In mice, the CD11b antigen is expressed on monocytes/macrophages and microglia. To a lower extent it is expressed on granulocytes, NK cells, CD5
+
B-1 cells, and subsets of dendritic cells. The monoclonal M1/70.15.11.5 antibody recognizes the human, mouse, and non-human primate CD11b antigen.

Alternative names

ITGAM, CR3A, Mac-1 α, MAC1A, MO1A, SLEB6, integrin αM

Detailed product information

Technical specifications

CloneM1/70.15.11.5
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Specieshuman, mouse, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD11b
Alternative names of antigenITGAM, CR3A, Mac-1 α, MAC1A, MO1A, SLEB6, integrin αM
Molecular mass of antigen [kDa]125
Distribution of antigenB cells, T cells, dendritic cells, granulocytes, monocytes, macrophages, NK cells
Entrez Gene ID3684
RRIDAB_2733615, AB_2726320, AB_2726045, AB_2726317, AB_2726042, AB_2726322, AB_2726047, AB_2726323, AB_2726048, AB_2726321, AB_2726046, AB_2726318, AB_2726043, AB_2733023, AB_2733024, AB_2726319, AB_2726044, AB_2727205, AB_2733614

References for CD11b Antibody, anti-human/mouse

Publications

  1. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice. J. Neurosci. 30(29): 9651-9658
  2. Liu, T. et al. (2014)
    Gr-1
    +
    CD11b
    +
    cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.
    Sci Rep 4: 4833

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