Clone:
REA789
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGAE, aM290, alpha-E1, alpha-M290, integrin alpha E

Extended validation for CD103 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA789
2E7++
Cells were incubated with an excess of purified unconjugated CD103 (REA789) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD103. Splenocytes from C57BL/6 mice were stained with CD103 antibodies and with a suitable counterstaining. As a control, CD103 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD103 (REA789). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD103 (REA789). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD103 (REA789). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD103 Antibody, anti-mouse, REAfinity™

Overview

Clone REA789 recognizes the mouse CD103 antigen, a 175 kDa large, type I transmembrane glycoprotein also known as αE integrin. CD103 associates non-covalently with integrin β7 thereby forming the heterodimeric integrin αEβ7. It mediates cell-cell contact and is involved in homing processes by binding to its ligand e-cadherin. CD103 is expressed on >90% of intestinal intraepithelial lymphocytes (IEL), epithelial T cells, and regulatory T cells. Due to its differential expression on dendritic cells from peripheral tissues, it is useful in identifying dendritic cell subsets from intestinal lamina propria, lung, and dermis.
Additional information: Clone REA789 displays negligible binding to Fc receptors

Alternative names

ITGAE, aM290, alpha-E1, alpha-M290, integrin alpha E

Detailed product information

Technical specifications

CloneREA789
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD103
Alternative names of antigenITGAE, aM290, alpha-E1, alpha-M290, integrin alpha E
Molecular mass of antigen [kDa]127
Distribution of antigenB cells, T cells, lymphocytes, leukemia cells, thymocytes, breast, pancreas, skin, small intestine, spleen
Entrez Gene ID16407
RRIDAB_2654366, AB_2654367, AB_2654368, AB_2654369, AB_2654370, AB_2654371, AB_2654372, AB_2654373, AB_2654374, AB_2654365

Resources for CD103 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD103 Antibody, anti-mouse, REAfinity™

Publications

  1. Sung, S. S. et al. (2006) A major lung CD103 alphaE-beta7 integrin-positive epithelial dendritic cell population expressing Langerin and tight junction proteins. J. Immunol. 176(9): 2161-2172
  2. Bogunovic, M. et al. (2009) Origin of the lamina propria dendritic cell network. Immunity 31(3): 513-525
  3. Ginhoux, F. et al. (2007)
    Blood-derived dermal Langerin
    +
    dendritic cells survey the skin in the steady state.
    J. Exp. Med. 204(13): 3133-3146

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