The Anti-Siglec-F MicroBeads, mouse have been developed for the positive selection or depletion of cells expressing mouse Siglec-F antigen from mouse peripheral blood (eosinophils, minor fraction of neutrophils) or mouse lung single-cell suspensions (eosinophils and alveolar macrophages).

Data and images for Anti-Siglec-F MicroBeads, mouse

Figures

Figure 1

Siglec-F
+
eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
Siglec-F
+
cells
View details

Figure 1

Siglec-F
+
eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Siglec-F
+
eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 2

Siglec-F
+
alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio
®
770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
Siglec-F
+
cells
View details

Figure 2

Siglec-F
+
alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio
®
770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 2

Siglec-F
+
alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio
®
770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-Siglec-F MicroBeads, mouse

Overview

The Anti-Siglec-F MicroBeads, mouse have been developed for the positive selection or depletion of cells expressing mouse Siglec-F antigen from mouse peripheral blood (eosinophils, minor fraction of neutrophils) or mouse lung single-cell suspensions (eosinophils and alveolar macrophages).

Detailed product information

Background information

Siglec-F, also known as Siglec-5, is a member of the sialic acid-binding immunoglobulin (Ig)-like lectins (Siglecs). The extracellular Ig domain binds specifically carbohydrate structures containing sialic acid residues. Siglec-F has, like most Siglecs, an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain and is discussed to play a role in eosinophil accumulation and survival. It is expressed in a tissue dependent manner on most eosinophils, but also found on alveolar macrophages and some neutrophils.

References for Anti-Siglec-F MicroBeads, mouse

Publications

  1. Zhang, M. et al. (2007)
    Defining the
    in vivo
    function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils.
    Blood 109(10): 4280-4287
  2. Guo, J. P. et al. (2011) Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. Am. J. Respir. Cell Mol. Biol. 44(2): 238-243
  3. Bochner, B. (2009) Siglec-8 on human eosinophils and mast cells, and Siglec-Fon murine eosinophils, are functionally related inhibitory receptors. Clin. Exp. Allergy 39(3): 317-324

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