Find the products and resources you are looking for!
Get in touch!
Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Tumors are heterogeneous and contain many types of irregular structures. Standard histological analysis and slide-based microscopic pathology offer insufficient prognostic value because of sampling limitations and the inability to observe specimens and biopsies in their native 3D structure. This limitation not only leads to an increased false-negative rate, but also lacks the information necessary to gain insights into the inner-mechanisms of tumors. 3D imaging with the UltraMicroscope provides a reliable, 3D histological analysis of large tissue samples (Hägerling, R. et al. , 2017; Nojima, S. et al., 2017; Noë, M. et al., 2018) and thereby a better understanding of complex 3D networks of vasculature systems, such as, the tumor lymphatic microvascularization. This helps us to understand the pathological nature of cancer and provides essential information that might improve the accuracy of cancer staging. Furthermore, as those structures may be affected by drugs, the UltraMicroscope could help analyze structural changes due to cancer therapy.
Further reading:
Glioblastoma multiforme restructures the topological connectivity of cerebrovascular networks
Hahn, A. et al., Scientific Reports. (2019) 9: 11757.
Correlated MRI and Ultramicroscopy (MR-UM) of brain tumors reveals vast heterogeneity of tumor infiltration and neoangiogenesis in preclinical models and human disease.
Breckwoldt, M.O. et al., Front. Neurosci. (2019) 12: 1004.
3D Imaging and Quantitative Analysis of Vascular Networks: A Comparison of Ultramicroscopy and Micro-Computed Tomography.
Epah, J. et al., Theranostics (2018) 8: 2117-2133. doi:10.7150/thno.22610
VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations.
Hägerling, R. et al., JCI insight (2017) 2: e93424.
CUBIC pathology: three-dimensional imaging for pathological diagnosis.
Nojima, S. et al., Scientific reports (2017) 7: 9269.
Immunolabeling of Cleared Human Pancreata Provides Insights into Three-Dimensional Pancreatic Anatomy and Pathology.
Noë, M. et al., The American journal of pathology (2018) 188: 1530-1535.
Tumor infiltrating lymphocytes (TILs) are immune cells that have moved from blood into a tumor to attack cancer cells. In cancer therapy, TILs are removed from a patient’s tumor and treated with substances activating the lymphocytes. Large numbers of these activated lymphocytes are then reinfused to the patient to boost the immune system’s ability to kill the cancer cells. In cancer research, localization, characterization and quantification of TILs is essential to develop efficient cancer therapies. For this purpose, the spatial information is required and can only be provided by 3D imaging of entire tumors. Together with efficient markers, the UltraMicroscope is the tool of choice for this application.
Further reading:
Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy.
Männ, L. et al., J Vis Exp. (2017) 123, e55450, doi:10.3791/55450
Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy.
Schulz-Kuhnt, A. et al., J Vis Exp. (2019) 146, e59043, doi:10.3791/59043
Locally renewing resident synovial macrophages provide a protective barrier for the joint.
Culemann, S. et al., Nature (2019) 572: 670–675.
Compartmentalized gut lymph node drainage dictates adaptive immune responses.
Esterházy, D. et al., Nature (2019) 569: 126–130.
Three-Dimensional Cross-Sectional Light-Sheet Microscopy Imaging of the Inflamed Mouse Gut.
Zundler S. et al., Gastroenterology. (2017) 153: 898-900.
CAR T cells are T cells that have been genetically engineered to express chimeric antigen receptors specifically directed towards antigens on a patient's tumor cells. There is great potential for this approach to improve patient-specific cancer therapy in a profound way. Identification of suitable antigens is challenging, as they must be highly expressed on the majority of the cancer cells, but largely absent on normal tissues. For this purpose, the spatial distribution of applied CAR T cells in tumor samples must be analyzed. Classic histological analysis and 2D microscopy are unable to provide a 3D representative overview of the heterogeneous tumor distribution relative to the applied CAR T cells. Therefore, volumetric 3D imaging of large tissues with the UltraMicroscope would be a huge asset in this research field.
Further reading:
In vivo labeling for ex vivo 3D tracking of CAR T cells.
Brauner, J. et al., Poster, MoBi Meeting 2019
Deep learning reveals cancer metastasis and therapeutic antibody targeting in whole body.
Pan, C. et al., bioRxiv (2019).
Correlated MRI and Ultramicroscopy (MR-UM) of brain tumors reveals vast heterogeneity of tumor infiltration and neoangiogenesis in preclinical models and human disease.
Breckwoldt, M.O. et al., Front. Neurosci. (2019) 12: 1004.
Compartmentalized gut lymph node drainage dictates adaptive immune responses.
Esterházy, D. et al., Nature (2019) 569: 126–130.
Metastasis is a major challenge in cancer management and treatment. Understanding this complex process and how lymphatic spread of cancer occurs is important for effective treatment. However, the detection of disseminated tumor cells and assessment of tumor targeting drugs within animal bodies has been challenging due to the lack of imaging technologies that are able to detect single cancer cells in whole mouse models. In fact, a single disseminated tumor cell left undetected could affect long-term prognosis of melanoma patients. This challenge has recently been addressed using the UltraMicroscope to identify and quantify micrometastasis below a size of 75 µm. In addition to the fast mapping of all tumor cells in optically cleared mice, this work demonstrated significant potential in optimizing antibody-based therapies (Pan, C. et al., 2019). Researchers were able to assess the efficiency of therapeutic antibodies known to reduce tumor burden in whole mouse models using the UltraMicroscope. The therapeutic antibody was labeled with an orthogonal fluorophore relative to the target antibody and injected into a mouse with induced metastasis. The multiplexing of both antibodies allowed quantification of cancer cells that were not recognized by the therapeutic antibody, thereby assessing its efficiency at a single metastasis level within the whole mouse model. Therefore, panoptic imaging with the UltraMicroscope provides not only a deeper level of structural and mechanistic insights into cancer, but also its potential therapies.
Further reading:
Deep learning reveals cancer metastasis and therapeutic antibody targeting in whole body.
Pan, C. et al., bioRxiv (2019).
Tumor uptake of anti-CD20 fabs depends on tumor perfusion.
Mendler, C.T. et al., J. Nuc. Med. (2016) 57: 1971–1977.
Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.