Miltenyi Biotec-tested panel 22 (MBTP 22)
This application protocol describes the quantitative analysis of human pluripotent stem cell (PSC) differentiation potential using flow cytometry. Human PSCs can easily be differentiated using the StemMACS™ Trilineage Differentiation Kit which enables directed and reproducible differentiation into ectodermal, mesodermal, and endodermal lineages ready for downstream applications, such as flow cytometric analysis.
The staining panel is based on a set of REAfinity™ Antibodies to ensure low background and high staining specificity. The panel consists of the three marker combinations (combination 1: CD144 and CD140b for mesoderm analysis; combination 2: CD184 and Sox17 for endoderm analysis; combination 3: PAX-6 and Sox2 for ectoderm analysis) and includes both surface and intracellular markers. Surface markers are stained first. Subsequently, cells are fixed and permeabilized for intracellular staining. Since the marker combination for ectoderm detection only includes intracellular markers, no surface staining is needed for this sample.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD144 (VE-Cadherin)||REA199||Mesoderm||FITC||525/50 (blue)|
|CD184 (CXCR4)||REA649||Endoderm||APC||655-730 (red)|
|Sox17||REA701||Endoderm||Vio® B515||525/50 (blue)|
|Propidium Iodide Solution||-||Viability||-||655-730 (blue)|
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
If not otherwise specified, all marks displayed on the Miltenyi Biotec Internet site and documents are subject to the trademark rights of Miltenyi Biotec, including each of Miltenyi Biotec's primary brands, its model name plates, and its corporate logos and emblems.
For copyright note and a complete list of trademarks visit www.miltenyibiotec.com/legalnotes.
Seems like you are coming from USA!
Do you want to visit our website in your country?