Find the products and resources you are looking for!
Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
In this application protocol, we describe an easy and fast method for the dissociation of skeletal muscle tissue from mouse and a subsequent isolation method that generates a highly pure population of satellite cells and minimizes bias in downstream applications caused by contamination with non-target cells.
Use the Skeletal Muscle Dissociation Kit to generate a single-cell suspension by combining mechanical dissociation with the gentleMACS™ Dissociator and enzymatic degradation of the extracellular matrix that maintains the structural integrity of the tissue. Follow the protocol of the kit data sheet.
Download data sheet
Isolate functional satellite cells by depleting non-target cells with the Satellite Cell Isolation Kit. The kit contains a cocktail of monoclonal antibodies conjugated with MACS® MicroBeads the magnetically label non-target cells. These are retained within the MACS Column, while the unlabeled satellite cells pass through during elution. Follow the protocol of the kit data sheet.
Download the data sheet
The following is a list of additional resources that may be of interest:
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
autoMACS, CliniMACS, the CliniMACS logo, CliniMACS Prodigy, CryoMACS, CytoMix, CytoStim, DendriMACS, ExiTron, ExpAct, FeraSpin, FeraTrack, GadoSpin, gentleMACS, LIFE 18, LIFE 21, MACS, the MACS logo, MACSductin, MACSelect, MACSfectin, MACSflex, MACSiBead, MACSiMAG, MACSmix, MACSprep, MACSQuant, MACSQuantify, MACSxpress, MidiMACS, MiniMACS, miRXplore, MultiMACS, NeuroBrew, NiraWave, OctoMACS, PepTivator, pMACS, PolySon, PrepProtect, QuadroMACS, REAfinity, REAlease, Rheo, StemMACS, StraightFrom, SuperAmp, SuperMACS, TexMACS, TheraSorb, thermoMACS, TransAct, Tyto, the Tyto logo, VarioMACS, Vio, Viobility, VioBlue, VioBright, VioGreen, Viscover, and µMACS are registered trademarks or trademarks of Miltenyi Biotec B.V. & Co. KG and/or its affiliates in various countries worldwide. Ficoll-Paque is a trademark of GE Healthcare companies. Vectofusin-1 is a registered trademark of Genethon. All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Copyright © 2019 Miltenyi Biotec B.V. & Co. KG and/or its affiliates. All rights reserved.
(Optional) ART. 1000 REACH™ pipet tips (Molecular BioProducts, Inc.) for removal of dissociated material from the closed C Tubes
|Column||Max. number of labeled cells||Max. number of total cells||Separator|
|LS||2×107||4×107||MidiMACS™, QuadroMACS™, |
VarioMACS, SuperMACS II
▲ Note: Column adapters are required to insert certain columns into the VarioMACS™ or SuperMACS™ II Separators. For details refer to the respective MACS Separator data sheet.
Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.