Immunophenotyping of SARS-CoV-2–specific B cells from human PBMCs using flow cytometry

Miltenyi Biotec-tested panel 42 (MBTP 42)

This application protocol for SARS-CoV-2–specific B cell immunophenotyping describes the analysis of SARS-CoV-2–specific B cells isolated from human PBMCs using recombinant SARS-CoV-2 spike proteins and flow cytometry. 


Antibody panel for SARS-CoV-2–specific B cell immunophenotyping

SpecificityClonePurposeFluorochromeDetection filter (nm) (laser)
CD19LT19Pan B cellsAPC-Vio® 770750 LP (red)
CD27M-T271Memory B cellsVio Bright FITC525/50 (violet)
IgAIS11-8E10Subset of memory B cellsVioGreen™525/50 (violet)
IgGIS11-3B2.2.3Subset of memory B cellsVioBlue®450/50 (violet)
IgMPJ2-22H3Subset of memory B cellsAPC655–730 (red)
Streptavidin-Spike-tetramerPE585/40 (blue)
Streptavidin-Spike-tetramerPE-Vio 770750 LP (blue)
7-AAD Staining Solution-Viability-655-730 (blue)
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