Immunophenotyping of CAR T cell effector cytokine production using flow cytometry

Miltenyi Biotec-tested panel 44 (MBTP 44)

This application protocol describes the characterization of CAR T cell functionality in terms of effector cytokine production upon antigen encounter using flow cytometry. To demonstrate the state-of-the-art panel, CAR T cells were co-cultured with antigen-expressing or antigen-negative target cells (GFP+) in presence of Brefeldin A. 



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