Treg Analysis Kits, anti-mouse, REAfinity™

Name: Treg Analysis Kits, anti-mouse, REAfinity™
Availability: InStock

Treg Analysis Kits, anti-mouse, REAfinity™

Applications:

FC

Product data and images for Treg Analysis Kits, anti-mouse, REAfinity™

Figures

Figure 1

Mouse lymph nodes were stained with CD4-VioBlue

^®

and CD25-APC antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-PE antibodies. Cells were analyzed by flow cytometry. Autofluorescent cell debris was excluded in an FL-2 versus FL-3 dot plot (data not shown). Gating was performed according to CD4 expression (except for the CD4/Anti-FoxP3 dot plot).

Figure 1

Mouse lymph nodes were stained with CD4-VioBlue

^®

and CD25-APC antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-PE antibodies. Cells were analyzed by flow cytometry. Autofluorescent cell debris was excluded in an FL-2 versus FL-3 dot plot (data not shown). Gating was performed according to CD4 expression (except for the CD4/Anti-FoxP3 dot plot).

Figure 1

Mouse lymph nodes were stained with CD4-VioBlue

^®

and CD25-APC antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3-PE antibodies. Cells were analyzed by flow cytometry. Autofluorescent cell debris was excluded in an FL-2 versus FL-3 dot plot (data not shown). Gating was performed according to CD4 expression (except for the CD4/Anti-FoxP3 dot plot).

Figure 2

Mouse lymph nodes were stained with CD4-VioBlue

^®

and CD25-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3 -Vio

^®

667 antibodies. Cells were analyzed by flow cytometry. Autofluorescent cell debris was excluded in an FL-2 versus FL-3 dot plot (data not shown). Gating was performed according to CD4 expression (except for the CD4/Anti-FoxP3 dot plot).

Figure 2

Mouse lymph nodes were stained with CD4-VioBlue

^®

and CD25-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3 -Vio

^®

667 antibodies. Cells were analyzed by flow cytometry. Autofluorescent cell debris was excluded in an FL-2 versus FL-3 dot plot (data not shown). Gating was performed according to CD4 expression (except for the CD4/Anti-FoxP3 dot plot).

Figure 2

Mouse lymph nodes were stained with CD4-VioBlue

^®

and CD25-PE antibodies. Cells were fixed, permeabilized, and stained with Anti-FoxP3 -Vio

^®

667 antibodies. Cells were analyzed by flow cytometry. Autofluorescent cell debris was excluded in an FL-2 versus FL-3 dot plot (data not shown). Gating was performed according to CD4 expression (except for the CD4/Anti-FoxP3 dot plot).

Specifications for Treg Analysis Kits, anti-mouse, REAfinity™

Overview

The Treg Detection Kits (CD4/CD25/FoxP3), mouse, have been developed for the convenient detection of CD4

⁺

CD25

⁺

FoxP3

⁺

regulatory T (Treg) cells. The kits include all required reagents for the analysis of mouse Treg cells by flow cytometry.

Detailed product information

Background information

Treg cells are a subset of T cells with the ability to suppress harmful immunological reactions to self and foreign antigens. Due to the lack of a specific marker for Treg cells, a combination of CD4, CD25, and FoxP3 is commonly used for their identification. The Treg Detection Kit (CD4/CD25/FoxP3) includes all reagents necessary for cell surface staining of CD4 and CD25, and intracellular staining of FoxP3 with a special FoxP3 Staining Buffer Set. The kit also includes an optimized protocol.

Resources for Treg Analysis Kits, anti-mouse, REAfinity™

Documents and Protocols

Brochures/posters

Regulatory T cell research

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for Treg Analysis Kits, anti-mouse, REAfinity™

Publications

Hori, S. et al. (2003) Control of regulatory T cell development by the transcription factor FoxP3. Science 299: 1057-1061

Data and images