TM4SF1 Antibody, anti-human, REAfinity™

Name: TM4SF1 Antibody, anti-human, REAfinity™
Availability: InStock

TM4SF1 Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA851

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

FC

Alternative names:

Transmembrane 4 L6 family member 1

Flow cytometry applications forTM4SF1 Antibody, anti-human, REAfinity™

APC
Biotin
FITC
PE
Vio Bright R720
Vio Bright V423

Conjugate:

APC

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-112-905, 130-112-716

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

A549 cells were stained with Anti-TM4SF1 antibodies or with the corresponding REA Control (S) antibodies (left peak). Flow cytometry was performed using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Extended validation for TM4SF1 Antibody, anti-human, REAfinity™

Specificity

Knockout validation

To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.

WT

KO

Fluorescence microscopy image of TM4SF1 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TM4SF1-PE (REA851, red) and counterstained with DRAQ5 (blue) as DNA stain.

Fluorescence microscopy image of TM4SF1 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TM4SF1-PE (REA851, red) and counterstained with DRAQ5 (blue) as DNA stain.

Overlay histogram showing flow cytometric analysis of TM4SF1 knockout cells. Wild type (red) and knockout cells (blue) were stained with TM4SF1-PE, Clone (REA851). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Overlay histogram showing flow cytometric analysis of TM4SF1 knockout cells. Wild type (red) and knockout cells (blue) were stained with TM4SF1-PE, Clone (REA851). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for TM4SF1 Antibody, anti-human, REAfinity™

Overview

Clone REA851 recognizes the human TM4SF1 antigen, also known as transmembrane 4 L6 family member 1. TM4SF1 is a member of the tetraspanin family, which members are involved in signal transduction events, important for the regulation of cell development, activation, growth, and motility. It is reported, that TM4SF1 suppresses the expression of CD63 and CD82, two molecules that inhibit cell mobility. TM4SF1 is highly expressed in lung, breast, colon, and ovarian carcinomas. It is also found on some endothelial cells.

Additional information: Clone REA851 displays negligible binding to Fc receptors.

Alternative names

Transmembrane 4 L6 family member 1

Detailed product information

Technical specifications

Clone	REA851
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (S), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	TM4SF1
Alternative names of antigen	Transmembrane 4 L6 family member 1
Molecular mass of antigen [kDa]	22
Distribution of antigen	endothelial cells, lung cancer cells, breast cancer cells, colorectal carcinoma cells
Entrez Gene ID	4071
RRID	AB_2654199, AB_2654200, AB_2654201, AB_2654202, AB_2654203, AB_2654204, AB_2654205, AB_2928932, AB_2928931, AB_2928930, AB_2928929, AB_2654198

Resources for TM4SF1 Antibody, anti-human, REAfinity™

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for TM4SF1 Antibody, anti-human, REAfinity™

Publications

Marken, J. S. et al. (1992) Cloning and expression of the tumor-associated antigen L6. Proc. Natl. Acad. Sci. U.S.A. 89(8): 3503-3507
Marken, J. S. et al. (1994) Membrane topology of the L6 antigen and identification of the protein epitope recognized by the L6 monoclonal antibody. J. Biol. Chem. 269(10): 7397-7401

Applications

Extended validation