TCR Vδ2 Antibody, anti-human, REAfinity™

Name: TCR Vδ2 Antibody, anti-human, REAfinity™
Availability: InStock

TCR Vδ2 Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA771

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

FC

Alternative names:

TCR VD2, TRDV2, hDV102S1

Flow cytometry applications forTCR Vδ2 Antibody, anti-human, REAfinity™

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 615
PE-Vio 770
PerCP-Vio 700
Vio Bright B515
Vio Bright R720
VioBlue
VioGreen

Conjugate:

APC

Recommended dilution:

1:50

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-111-128, 130-111-011

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR Vδ2 antibodies or with the corresponding REA Control (S) antibodies (left image) as well as with CD3 antibodies. Flow cytometry was performed using the MACSQuant^® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Extended validation for TCR Vδ2 Antibody, anti-human, REAfinity™

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with REA771
B6	++
IMMU 389	-
15D	-
123R3	++
Cells were incubated with an excess of purified unconjugated TCR Vδ2 (REA771) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for TCR Vδ2. Lysed whole blood was stained with TCR Vδ2 antibodies and with a suitable counterstaining. As a control, TCR Vδ2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for TCR Vδ2. Lysed whole blood was stained with TCR Vδ2 antibodies and with a suitable counterstaining. As a control, TCR Vδ2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for TCR Vδ2. Lysed whole blood was stained with TCR Vδ2 antibodies and with a suitable counterstaining. As a control, TCR Vδ2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for TCR Vδ2. Lysed whole blood was stained with TCR Vδ2 antibodies and with a suitable counterstaining. As a control, TCR Vδ2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for TCR Vδ2. Lysed whole blood was stained with TCR Vδ2 antibodies and with a suitable counterstaining. As a control, TCR Vδ2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using TCR Vδ2 (REA771). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using TCR Vδ2 (REA771). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCR Vδ2 Antibody, anti-human, REAfinity™

Overview

Clone REA771 recognizes the Vδ2 TCR chain on human Vγ9Vδ2 T cells, which are the major γ/δ T cell subset in humans. Activated Vδ2 T lymphocytes display strong cytotoxicity against various tumor cells and produce a number of cytokines, including TNF-α and IFN-γ.

Additional information: Clone REA771 displays negligible binding to Fc receptors.

Alternative names

TCR VD2, TRDV2, hDV102S1

Detailed product information

Technical specifications

Clone	REA771
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (S), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	TCR Vδ2
Alternative names of antigen	TCR VD2, TRDV2, hDV102S1
Molecular mass of antigen [kDa]	11
Distribution of antigen	T cells
Entrez Gene ID	28517
RRID	AB_2653973, AB_2653974, AB_2653975, AB_2653976, AB_2653977, AB_2653978, AB_2653979, AB_2653980, AB_2653981, AB_2653982, AB_2653983, AB_2653984, AB_2653985, AB_2653986, AB_2653969, AB_2653970, AB_2653971, AB_2653972

Resources for TCR Vδ2 Antibody, anti-human, REAfinity™

Certificates

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References for TCR Vδ2 Antibody, anti-human, REAfinity™

Publications

Rincon-Orozco, B. et al. (2005) Activation of V gamma 9V delta 2 T cells by NKG2D. J Immunol 175(4): 2144-2151
Scotet, E. et al. (2005) Tumor recognition following Vgamma9Vdelta2 T cell receptor interactions with a surface F1-ATPase-related structure and apolipoprotein A-I. Immunity 22(1): 71-80

Applications

Extended validation