Clone:
REA640
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
SYND4, Ryudocan core protein, AA959608, AW108331, S4

Extended validation for Syndecan-4 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA640
KY/8.2++
112-
Cells were incubated with an excess of purified unconjugated Syndecan-4 (REA640) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Syndecan-4. C57BL/6 splenocytes were stained with Syndecan-4 antibodies and with a suitable counterstaining. As a control, Syndecan-4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Syndecan-4. C57BL/6 splenocytes were stained with Syndecan-4 antibodies and with a suitable counterstaining. As a control, Syndecan-4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Syndecan-4. C57BL/6 splenocytes were stained with Syndecan-4 antibodies and with a suitable counterstaining. As a control, Syndecan-4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Syndecan-4. C57BL/6 splenocytes were stained with Syndecan-4 antibodies and with a suitable counterstaining. As a control, Syndecan-4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using Syndecan-4 (REA640). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using Syndecan-4 (REA640). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using Syndecan-4 (REA640). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Syndecan-4 Antibody, anti-mouse, REAfinity™

Overview

Clone REA640 recognizes the mouse syndecan-4 antigen, also known as SYND4 or ryudocan core protein. Syndecan-4 belongs to the cell surface syndecan proteoglycan family, which binds a variety of soluble and insoluble extracellular effectors. Syndecan-4 is able to bear heparan sulfate and is an important regulator of bFGF signaling. It is expressed ubiquitously, but mainly in liver, kidney, and lung. It is highly expressed on activated B lymphocytes.
Additional information: Clone REA640 displays negligible binding to Fc receptors.

Alternative names

SYND4, Ryudocan core protein, AA959608, AW108331, S4

Detailed product information

Technical specifications

CloneREA640
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenSyndecan-4
Alternative names of antigenSYND4, Ryudocan core protein, AA959608, AW108331, S4
Molecular mass of antigen [kDa]19
Entrez Gene ID20971
RRIDAB_2653636, AB_2653637, AB_2653640, AB_2653641, AB_2653642, AB_2653643, AB_2653644, AB_2653645, AB_2819381

References for Syndecan-4 Antibody, anti-mouse, REAfinity™

Publications

  1. Tsuzuki, S. et al. (1997) Molecular cloning, genomic organization, promoter activity, and tissue-specific expression of the mouse ryudocan gene. J. Biochem. 122(1): 17-24
  2. Fitzgerald, M. L. et al. (2000) Shedding of syndecan-1 and -4 ectodomains is regulated by multiple signaling pathways and mediated by a TIMP-3-sensitive metalloproteinase. J. Cell Biol. 148(4): 811-824
  3. Gao, Y. et al. (2000) Synectin, syndecan-4 cytoplasmic domain binding PDZ protein, inhibits cell migration. J. Cell. Physiol. 184(3): 373-379

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