Name: StraightFrom® Whole Blood CD15 MicroBeads, human
Availability: InStock

Product data and images for StraightFrom
^®
Whole Blood CD15 MicroBeads
, human

Figures

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

Whole Blood CD15 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD15-FITC, CD66b-PE, as well as CD45‑VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

After separation

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for StraightFrom
^®
Whole Blood CD15 MicroBeads
, human

Overview

StraightFrom

^®

Whole Blood CD15 MicroBeads have been developed for rapid positive selection of CD15

⁺

cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis.

Detailed product information

Background information

StraightFrom

^®

Whole Blood CD15 MicroBeads recognize the carbohydrate structure 3-fucosyl-N-acetyllactosamine (3-FAL), also designated Lewis X. The CD15 antigen is expressed on neutrophils and eosinophils but not on basophils and lymphocytes.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom

^®

Whole Blood CD15 MicroBeads to be subsequently separated with the autoMACS

^®

Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

StraightFrom

^®

Whole Blood MicroBeads allow the fast isolation of CD15

⁺

cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS

^®

Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD15

⁺

cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation

^1,2,3

Columns

autoMACS

^®

Columns or Whole Blood Columns.

Resources for StraightFrom
^®
Whole Blood CD15 MicroBeads
, human

Documents and Protocols

Brochures/posters

StraightFrom® MicroBeads - Cell isolation directly from blood products

App notes/customer reports

Willasch et al. (2012) Enrichment of cell subpopulations by MACS® Technology for PCR-based chimerism analysis.

References for StraightFrom
^®
Whole Blood CD15 MicroBeads
, human

Publications

Meyer, R. G. et al. (2007) Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell-depleted reduced-intensity transplantation. Blood 109: 374-382
Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
Adams, S. et al. (2004) Cell lineage-specific chimerism in posthematopoietic stem cell transplant patients. MACS&more 8(2): 16
Willasch, A. et al. (2010) Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis. Bone Marrow Transplant. 45: 181-189

Data and images

Data and images for StraightFrom
^®
Whole Blood CD15 MicroBeads
, human

Figures

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

After separation

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for StraightFrom
^®
Whole Blood CD15 MicroBeads
, human

Overview

StraightFrom

^®

Whole Blood CD15 MicroBeads have been developed for rapid positive selection of CD15

⁺

cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis.

Detailed product information

Background information

StraightFrom

^®

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom

^®

Whole Blood CD15 MicroBeads to be subsequently separated with the autoMACS

^®

Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

StraightFrom

^®

Whole Blood MicroBeads allow the fast isolation of CD15

⁺

cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS

^®

Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD15

⁺

cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation

^1,2,3

Columns

autoMACS

^®

Columns or Whole Blood Columns.

Resources

Resources for StraightFrom
^®
Whole Blood CD15 MicroBeads
, human

Documents and Protocols

Brochures/posters

References for StraightFrom
^®
Whole Blood CD15 MicroBeads
, human

Publications

Meyer, R. G. et al. (2007) Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell-depleted reduced-intensity transplantation. Blood 109: 374-382
Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
Adams, S. et al. (2004) Cell lineage-specific chimerism in posthematopoietic stem cell transplant patients. MACS&more 8(2): 16
Willasch, A. et al. (2010) Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis. Bone Marrow Transplant. 45: 181-189

Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

StraightFrom® Whole Blood CD15 MicroBeads, human

StraightFrom® Whole Blood CD15 MicroBeads, human

Product data and images for StraightFrom® Whole Blood CD15 MicroBeads, human

Figures

Figure 1