StemMACS iPS-Brew XF is a xeno-free cell culture medium for the maintenance of human ES and iPS cells under feeder-free conditions.

Data and images for StemMACS™ iPS-Brew XF, human

Figures

Figure 1

Pluripotent stem cells cultured in StemMACS iPS-Brew XF display typical colony morphology.
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Figure 1

Pluripotent stem cells cultured in StemMACS iPS-Brew XF display typical colony morphology.
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Figure 1

Pluripotent stem cells cultured in StemMACS iPS-Brew XF display typical colony morphology.

Figure 2

Cells cultured in StemMACS iPS-Brew XF maintain full pluripotent differentiation potential and can form teratomas containing derivatives of all three germ layers.
Endoderm
Ectoderm
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Figure 2

Cells cultured in StemMACS iPS-Brew XF maintain full pluripotent differentiation potential and can form teratomas containing derivatives of all three germ layers.
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Figure 2

Cells cultured in StemMACS iPS-Brew XF maintain full pluripotent differentiation potential and can form teratomas containing derivatives of all three germ layers.
Mesoderm
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Figure 2

Cells cultured in StemMACS iPS-Brew XF maintain full pluripotent differentiation potential and can form teratomas containing derivatives of all three germ layers.

Figure 3

Human iPS cells cultured in StemMACS iPS-Brew XF show high expression of pluripotency markers.
Clone 1
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Figure 3

Human iPS cells cultured in StemMACS iPS-Brew XF show high expression of pluripotency markers.
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Figure 3

Human iPS cells cultured in StemMACS iPS-Brew XF show high expression of pluripotency markers.
Clone 2
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Figure 3

Human iPS cells cultured in StemMACS iPS-Brew XF show high expression of pluripotency markers.
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Figure 3

Human iPS cells cultured in StemMACS iPS-Brew XF show high expression of pluripotency markers.

Specifications for StemMACS™ iPS-Brew XF, human

Overview

StemMACS iPS-Brew XF is a xeno-free cell culture medium for the maintenance of human ES and iPS cells under feeder-free conditions.

Detailed product information

Background information

StemMACS iPS-Brew XF is a xeno-free cell culture media formulation for the maintenance and expansion of human pluripotent stem cells under feeder-free conditions. The formulation supports rapid adaption of feeder-based cell cultures to a feeder-free environment and is compatible with commonly used cell attachment matrices, e.g. Matrigel or Laminin-521. StemMACS iPS-Brew XF enables robust and efficient expansion of human embryonic stem (ES) cells or induced pluripotent stem (iPS) cells over multiple passages while maintaining a pluripotent phenotype as well as pluripotent differentiation potential. StemMACS iPS-Brew XF allows rapid culture re-initiation of pluripotent stem cell cultures after cryopreservation.

Applications

  • Culture of human ES or iPS cells under xeno- and feeder-free conditions
  • Rapid and easy adaption of feeder-based cell cultures to a feeder-free environment
  • Rapid culture initiation after cryopreservation

References for StemMACS™ iPS-Brew XF, human

Publications

  1. Kirkeby, A. et al. (2017) Predictive markers guide differentiation to improve graft outcome in clinical translation of hESC-based therapy for Parkinson’s Disease. Cell Stem Cell 20: 1-14
  2. Lorenz, C. et al. (2017) Human iPSC-derived neural progenitors are an effective drug discovery model for neurological mtDNA disorders. Cell Stem Cell 20(5): 659-674
  3. Mitzelfeld, K. et al. (2016) The human 343delT HSPB5 chaperone associated with early-onset skeletal myopathy causes defects in protein solubility. J. Biol. Chem. 291(29): 14939-14953
  4. Giobbe, G.G. et al. (2015) Functional differentiation of human pluripotent stem cells on a chip. Nat. Methods 12: 637-640
  5. Park, C.-Y. et al. (2016) Three-dimensional imaging of solvent-cleared organs using 3DISCO. Nat. Protoc. 11: 2154-2169
  6. Solbrant, S. et al. (2017) Generation of high-purity human ventral midbrain dopaminergic progenitors for in vitro maturation and intracerebral transplantation Nat. Protoc. 12(9): 1962-1979
  7. Es-Salah-Lamoureux, Z. et al. (2016) HIV-Tat induces a decrease in IKr and IKs via reduction in phosphatidylinositol-(4,5)-bisphosphate availability. J. Mol. Cell. Cardiol. 99: 1-13
  8. Si-Tayeb, K. et al. (2016) Urine‑sample‑derived human induced pluripotent stem cells as a model to study PCSK9‑mediated autosomal dominant hypercholesterolemia. Dis Model Mech 9(1): 81-90

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