Flow cytometric comparison of different clones for Anti-Siglec-8. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with Anti-Siglec-8 antibodies and with a suitable counterstaining. As a control, Anti-Siglec-8 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-Siglec-8. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with Anti-Siglec-8 antibodies and with a suitable counterstaining. As a control, Anti-Siglec-8 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-Siglec-8. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with Anti-Siglec-8 antibodies and with a suitable counterstaining. As a control, Anti-Siglec-8 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-Siglec-8. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with Anti-Siglec-8 antibodies and with a suitable counterstaining. As a control, Anti-Siglec-8 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Comparison of staining pattern on non-fixed and fixed cells using Siglec-8 (REA1045). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using Siglec-8 (REA1045). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.