PTK7 (CCK-4) Antibody, anti-human, REAfinity™
Clone:
REA836
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
CCK-4, CCK4

Extended validation for PTK7 (CCK-4) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of PTK7 (CCK-4) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with PTK7 (CCK-4)-PE (REA836, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of PTK7 (CCK-4) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with PTK7 (CCK-4)-PE (REA836, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of PTK7 (CCK-4) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with PTK7 (CCK-4)-PE (REA836, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of PTK7 knockout cells. Wild type (red) and knockout cells (blue) were stained with PTK7-PE, clone (REA836). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of PTK7 knockout cells. Wild type (red) and knockout cells (blue) were stained with PTK7-PE, clone (REA836). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for PTK7 (CCK-4) Antibody, anti-human, REAfinity™

Overview

Clone REA836 recognizes human protein tyrosine kinase-7 (PTK7). PTK7, also known as colon carcinoma kinase-4 (CCK-4), is a receptor protein tyrosine kinase (RPTK)–like molecule that contains a catalytically inactive tyrosine kinase domain. PTK7 was identified to be important for neural development as a regulator of planar cell polarity, being required for convergent extension movement and neural tube closure. Using the Anti-PTK7 (CCK-4) antibody, PTK7 expression was recently analyzed on blood and bone marrow cells. PTK7 was shown to be expressed on plasmacytoid dendritic cells, CD141 (BDCA-3)
high
type-2 myeloid dendritic cells, and CD34
+
hematopoietic progenitor cells (HPCs). PTK7 was detected on early (CD34
+
CD133
+
) and late (CD34
+
CD133
–
) HPCs. In tonsils, PTK7 was also found on some T cells.
Additional information: Clone REA836 displays negligible binding to Fc receptors.

Alternative names

CCK-4, CCK4

Detailed product information

Technical specifications

CloneREA836
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenPTK7 (CCK-4)
Alternative names of antigenCCK-4, CCK4
Molecular mass of antigen [kDa]115
Distribution of antigendendritic cells, bone marrow
Entrez Gene ID5754
RRIDAB_2653289, AB_2653290, AB_2653291, AB_2653292, AB_2653293, AB_2653294, AB_2653295, AB_2653296, AB_2653297, AB_2653298

Resources for PTK7 (CCK-4) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for PTK7 (CCK-4) Antibody, anti-human, REAfinity™

Publications

  1. Mossie, K. et al. (1995) Colon carcinoma kinase-4 defines a new subclass of the receptor tyrosine kinase family. Oncogene 11: 2179-2184
  2. Park, S. K. et al. (1996) Characterization of the human full-length PTK7 cDNA encoding a receptor protein tyrosine kinase-like molecule closely related to chick KLG. J. Biochem. 119(2): 235-239
  3. Katoh, M. and Katoh, M. (2007) Comparative integromics on non-canonical WNT or planar cell polarity signaling molecules: transcriptional mechanism of PTK7 in colorectal cancer and that of SEMA6A in undifferentiated ES cells. Int. J. Mol. Med. 20: 405-409
  4. Xiao, Z. et al. (2008) Cell-specific internalization study of an aptamer from whole cell selection. Chemistry 14: 1769-1775
  5. Lu, X. et al. (2004) PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates. Nature 430: 93-98

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