Perforin Antibody, anti-human

Name: Perforin Antibody, anti-human
Availability: InStock

Perforin Antibody, anti-humanExtended Validation

Clone:

delta G9

Type of antibody:

Primary antibodies

Isotype:

mouse IgG2bκ

Applications:

ICFC

Alternative names:

PRF1, FLH2, HPLH2, P1, PFN1, PFP

Intracellular flow cytometry applications forPerforin Antibody, anti-human

APC
Biotin
FITC
PE
VioBlue

Conjugate:

APC

Recommended dilution:

1:10

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-096-569

Protocols:

Intracellular flow cytometry staining protocol (Inside Stain Kit 1:10, protocol A)

Description:

Fixed and permeabilized human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Perforin antibodies as well as with CD8 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Gating was performed on lymphocytes according to scatter properties. Cell debris and dead cells were excluded from the analysis based on scatter signals and in a fluorescence 3 versus fluorescence 4 dot plot.

Extended validation for Perforin Antibody, anti-human

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Perforin Antibody, anti-human

Overview

The clone delta G9 reacts with human perforin, a 70 kDa cytolytic protein, which is expressed by cytotoxic T lymphocytes and natural killer (NK) cells, and which is stored in cytoplasmic granules. Upon cell activation perforin is released and functions as one of the major effector molecule to mediate killing of target cells.

Alternative names

PRF1, FLH2, HPLH2, P1, PFN1, PFP

Detailed product information

Technical specifications

Clone	delta G9
Clonality	monoclonal
Isotype	mouse IgG2bκ
Isotype control	Isotype Control Antibody, mouse IgG2b
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	Perforin
Alternative names of antigen	PRF1, FLH2, HPLH2, P1, PFN1, PFP
Molecular mass of antigen [kDa]	59
Entrez Gene ID	5551
RRID	AB_2819519, AB_2811588, AB_2811567, AB_2653179, AB_10839415, AB_2653182, AB_2819554

Resources for Perforin Antibody, anti-human

Certificates

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Applications

Extended validation