Pan T Cell MicroBeads, rat

Name: Pan T Cell MicroBeads, rat
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Pan T Cell MicroBeads, rat

PanT Cell MicroBeads have been developed for the positive selection or depletion of rat T cells from blood, lymphoid organs, and cell suspensions of non-lymphoid tissues, such as lung or ovary.

Product data and images for Pan T Cell MicroBeads, rat

Figures

Figure 1

Isolation of T cells from rat spleen using Pan T Cell MicroBeads, a MidiMACS™ Separator, and an LS Column.

A:	B:
Spleen cells before separation	T cell–depleted fraction
View details Pan T Cell MicroBeads, rat Figure 1 Isolation of T cells from rat spleen using Pan T Cell MicroBeads, a MidiMACS™ Separator, and an LS Column.	View details Pan T Cell MicroBeads, rat Figure 1 Isolation of T cells from rat spleen using Pan T Cell MicroBeads, a MidiMACS™ Separator, and an LS Column.
C:
Isolated T cells
View details Pan T Cell MicroBeads, rat Figure 1 Isolation of T cells from rat spleen using Pan T Cell MicroBeads, a MidiMACS™ Separator, and an LS Column.

Specifications for Pan T Cell MicroBeads, rat

Overview

PanT Cell MicroBeads have been developed for the positive selection or depletion of rat T cells from blood, lymphoid organs, and cell suspensions of non-lymphoid tissues, such as lung or ovary.

Detailed product information

Background information

The OX52 surface antigen recognized by the Pan T Cell MicroBeads is expressed in a lineage-specific manner on thymocytes and T cells. T cells expressing T cell receptor (TCR) α/β as well as TCRγ/δ are recognized. The OX52 antigen is expressed by approximately 1% of bone marrow cells, 99% of thymocytes, 60% of leukocytes in thoracic duct, 60% of lymph node cells, and 30% of splenocytes. The antigen is also weakly expressed on NK cells, which can be distinguished from T cells due to their lack of CD3 or CD5 expression.

Downstream applications

T cells isolated with Pan T Cell MicroBeads can be used for

in vitro

and

in vivo

studies on T cell migration and differentiation in the context of autoimmune disease or alloreactivity. They were also used in adoptive transfer experiments to study autoimmunity or induction of tolerance after xenogeneic thymus transplantation.

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Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

References for Pan T Cell MicroBeads, rat

Publications

Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J Immunol 170: 5936-5946

Data and images