The Mo-DC Generation Toolbox comprises reagents for magnetic cell separation of CD14
+
monocytes and the subsequent differentiation and maturation to monocyte-derived dendritic cells (DCs). Large numbers of Mo-DCs can be generated
ex vivo
from purified monocytes (easily isolated from bone marrow, cord blood, peripheral blood, or peripheral blood mononuclear cells (PBMCs)). The excellent purity and recovery of selected monocytes offer a standardized and consistent cell source. The Mo-DC Differentiation Medium contains interleukin-4/GM-CSF. Mo-DCs can easily be matured  with the premium grade

Data and images for Mo-DC Generation Toolbox, human

Figures

Figure 1

Flow cytometric analysis of
in vitro
generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using culturing media supplemented with TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.
A:
B:
View details

Figure 1

Flow cytometric analysis of
in vitro
generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using culturing media supplemented with TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.
View details

Figure 1

Flow cytometric analysis of
in vitro
generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using culturing media supplemented with TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.
C:
View details

Figure 1

Flow cytometric analysis of
in vitro
generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using culturing media supplemented with TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.

Specifications for Mo-DC Generation Toolbox, human

Overview

The Mo-DC Generation Toolbox comprises reagents for magnetic cell separation of CD14
+
monocytes and the subsequent differentiation and maturation to monocyte-derived dendritic cells (DCs). Large numbers of Mo-DCs can be generated
ex vivo
from purified monocytes (easily isolated from bone marrow, cord blood, peripheral blood, or peripheral blood mononuclear cells (PBMCs)). The excellent purity and recovery of selected monocytes offer a standardized and consistent cell source. The Mo-DC Differentiation Medium contains interleukin-4/GM-CSF. Mo-DCs can easily be matured  with the premium grade TNF-α provided with the kit. The lot-to-lot consistency and standardized protocol we offer enable for effective differentiation of immature and mature Mo-DCs.

Detailed product information

Downstream applications

For the isolation of monocytes from PBMCs and subsequent differentiation of immature and mature monocyte-derived dendritic cells
in vitro
. Common uses for Mo-DCs are cellular vaccine development, antigen-specific T cell stimulation, as well as toxicity and hypersensitivity testing.

Columns

MS, LS, or autoMACS
®
Columns.

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