Reliability of the µMACS SuperAmp protocol using cell lines. A serial dilution of 1 to 10,000 Jurkat cells was generated and each dilution was independently processed using the µMACS SuperAmp Kit. The amplified and Cy5-labeled cDNA from Jurkat cells was hybridized in a two-color microarray experiment against amplified and Cy3-labeled cDNA from 1000 Raji cells.
Each experiment was repeated twice and the independently amplified samples were hybridized on PIQOR Immunology Microarrays. A) Pearson correlation coefficients (r) of independent repeats. B) Hierarchical clustering with no obvious grouping of repeats or cell numbers.