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Human peripheral blood mononuclear cells (PBMCs) were fixed and permeabilized using the Cell Signaling Buffer Set A . Cells were then stained with CD20 Cytoplasmic antibodies or with the corresponding REA Control (I) antibodies (left image) as well as with CD19 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals.
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