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Human peripheral blood mononuclear cells (PBMCs), either left unstimulated (left peak) or stimulated with 50 nM Phorbol 12-myristate 13-acetate (PMA) for 30 minutes at 37 °C, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were stained with Anti-S6 pS240 antibodies and analyzed by flow cytometry using the MACSQuant®
cells were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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