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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (right peak) or stimulated with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) for 24 hours. The cells were fixed and permeabilized with the Cell Signaling Buffer Set A and stained with Anti-PKCα antibodies. Cells were then analyzed by flow cytometry using the MACSQuant®
Analyzer. Lymphocytes were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals. As shown in the histogram, PMA stimulation of lymphocytes results in decreased PKCα expression.
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