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Splenocytes from C57BL/6 mice were stimulated with CD3 and CD28 antibodies for 24 hours. Cells were fixed, permeabilized, and stained with Anti-Notch1 antibodies or with the corresponding REA Control (I) antibodies (left peak). Flow cytometry was performed using the MACSQuant®
cells were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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