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HeLa cells, overnight serum starved and either left unstimulated (left peak) or stimulated with 150 nM calyculin A for 30 minutes, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were then stained with Anti-MEK1 pS298 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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