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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
T cells were enriched (CD4+
T Cell Isolation Kit II, mouse) from spleen cells of a BALB/c mouse. Cells were either left unstimulated (left image) or stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of recombinant TGF-beta, recombinant IL-4, recombinant IL-2, and neutralizing Anti-IL-6 antibody, Anti- IFN-γ antibody and Anti-IL-12 antibody. On day 5, brefeldin A was added for 4 hours. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-9 antibodies, as well as with CD4-FITC. Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.
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