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A mixture of splenocytes from BALB/c mice (left peak) and C57BL/6 mice was stained with Anti-H-2Db
antibodies as well as with Anti-Ter-119 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant®
cells were pregated for the analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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