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Single-cell suspensions from mouse or rat spleen were prepared using the gentleMACS™ Dissociator. CD90.1+
T cells were isolated from the single-cell suspensions using the CD90.1 MicroBeads, an autoMACS®
Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies. Mouse cells were stained with CD45-FITC, CD45R(220)-PE-Vio®
770 and CD90.1-VioBlue®
(A, B) and rat cells were stained with CD90.1-VioBlue (C, D). Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.
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