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plasma cells were isolated from human bone marrow using the Plasma Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, and two MS Columns and a MiniMACS™ Separator. Aliquots of the different cell fractions were fluorescently stained with CD19-APC and CD38-FITC for identification of plasma cells.
Bone marrow before separation
Pre-enriched plasma cells after depletion of non-plasma cells
Enriched CD38+ plasma cells
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