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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Isolation of germinal center B cells from mouse SRBC-immunized spleen using the Germinal Center B Cell (PNA) MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD19-PE-Vio®
770, CD38-APC, and CD95-PE and analyzed by flow cytometer using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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