Human SHH (C24II)

Name: Human SHH (C24II)
Availability: InStock

Human SHH (C24II)

Recombinant Human SHH (Sonic Hedgehog; or C24II) can promote growth and differentiation of embryonic stem cells, adult mesenchymal stem cells, and cells within the neuronal lineage. The morphogenetic cytokine is crucial for embryonic development, as it is involved in the patterning of the central nervous system, somites, and limbs. Recombinant Human SHH is ideal for use in cell culture, differentiation studies, and functional assays.

Product data and images for Human SHH (C24II)

Figures

Figure 1

Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.

TH (tyrosine hydroxylase) ⁺ cells	TuJ1 (neuron-specific β-III tubulin) ⁺ cells	GAD67 (glutamate decarboxylase) ⁺ cells
View details Human SHH (C24II) Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.	View details Human SHH (C24II) Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.	View details Human SHH (C24II) Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.

Figure 2

**Human SHH (C24II) activity assay.**
The biological activity of Human SHH (C24II) is determined by alkaline phosphatase assay using mouse C3H10T1/2 fibroblasts.

Figure 2

Human SHH (C24II) activity assay.

The biological activity of Human SHH (C24II) is determined by alkaline phosphatase assay using mouse C3H10T1/2 fibroblasts.

Figure 3

**SDS-PAGE of Human SHH (C24II), premium grade**
under reduced (R) and non-reduced (NR) conditions.

Figure 3

SDS-PAGE of Human SHH (C24II), premium grade

under reduced (R) and non-reduced (NR) conditions.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human SHH (C24II), premium grade. The peak corresponds to the calculated molecular mass of 19683 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human SHH (C24II), premium grade. The peak corresponds to the calculated molecular mass of 19683 Da.

Specifications for Human SHH (C24II)

Overview

Recombinant Human SHH (Sonic Hedgehog; or C24II) can promote growth and differentiation of embryonic stem cells, adult mesenchymal stem cells, and cells within the neuronal lineage. The morphogenetic cytokine is crucial for embryonic development, as it is involved in the patterning of the central nervous system, somites, and limbs. Recombinant Human SHH is ideal for use in cell culture, differentiation studies, and functional assays.

Applications

Human SHH (C24II) can be used for a variety of applications including:

Expansion or differentiation of various cell types, mainly human and mouse embryonic stem cells, but also adult stem cells and mesenchymal cells.

Detailed product information

Background information

Sonic hedgehog (SHH) is involved in a wide variety of embryonic developmental events, including neurogenesis, limb development, hematopoiesis, and gut formation. Besides their role as morphogens, hedgehog family members have also been shown to act as mitogens, cell survival factors, and axon guidance factors and to influence tissue regeneration within the adult. SHH is synthesized as a precursor, which is autocatalytically cleaved into a 19 kDa N-terminal domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N is responsible for the signaling activity of hedgehog proteins, while SHH-C acts as a cholesterol transferase and covalently attaches a cholesterol molecule to SHH-N. The lipid-modified SHH-N displays enhanced activity. Human SHH (C24II) corresponds to the mature SHH-N domain and carries a Cys to Ile-Ile substitution resulting in a lipophilic moiety and therefore an increased activity.

Biological activity

Alkaline phosphatase production by mouse C3H10T1/2 fibroblasts
research grade: ≥ 1×
10
³
U/mg
premium grade: ≥ 2×
10
³
U/mg
We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human SHH (C24II)

Documents and Protocols

Background information

Premium-grade cytokine benefits

App notes/customer reports

How to switch to optimal activity-based cytokine dosing

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for Human SHH (C24II)

Publications

Nakamura, T. et al. (1997) Induction of osteogenic differentiation by hedgehog proteins. Biochem. Biophys. Res. Commun. 237: 465-469
Fukusumi, H. et al. (2021) Alpha-synuclein dynamics in induced pluripotent stem cell-derived dopaminergic neurons from a Parkinson's disease patient (PARK4) with SNCA triplication. FEBS Open Bio. 11(2): 354-366
Liu, L. et al. (2019) Protection of ZIKV infection-induced neuropathy by abrogation of acute antiviral response in human neural progenitors. Cell Death Differ. 26(12): 2607
Costa-Besada, M. A. et al. (2018) Paracrine and Intracrine Angiotensin 1-7/Mas Receptor Axis in the Substantia Nigra of Rodents, Monkeys, and Humans. Mol Neurobiol 55(7): 5847
Chen, Z. et al. (2018) Genetic Engineering of Human Embryonic Stem Cells for Precise Cell Fate Tracing during Human Lineage Development. Stem Cell Reports 11(5): 1257
Bell, S. et al. (2017) A Rapid Pipeline to Model Rare Neurodevelopmental Disorders with Simultaneous CRISPR/Cas9 Gene Editing. Stem Cells Transl Med 6(3): 886-896

Data and images