Human SCF

Name: Human SCF
Availability: InStock

Human SCF

Alternative names:

c-kit ligand, steel factor, MGF

Product data and images for Human SCF

Figures

Figure 1

**Human SCF activity assay.**
The biological activity of Human SCF is determined by proliferation assay using TF-1 cells.

Figure 1

Human SCF activity assay.

The biological activity of Human SCF is determined by proliferation assay using TF-1 cells.

Figure 2

**SDS-PAGE of Human SCF, premium grade**
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human SCF, premium grade

under reduced (R) and non-reduced (NR) conditions.

Figure 3

**Human SCF biological activity.**
Activity of Human SCF, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 3

Human SCF biological activity.

Activity of Human SCF, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 4

Mass spectrometry analysis (ESI-MS) of Human SCF, premium grade. The peak corresponds to the calculated molecular mass of 18585 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human SCF, premium grade. The peak corresponds to the calculated molecular mass of 18585 Da.

Specifications for Human SCF

Overview

Recombinant human SCF (stem cell factor) can stimulate growth and activation of mast cells, as well as expansion of hematopoietic stem cells and myeloid, erythroid, or lymphoid progenitors. The c-Kit receptor transmits the SCF signal, which acts at different levels of embryonic and adult hematopoiesis. For successful use in cell culture, differentiation studies, and functional assays, the recombinant protein human SCF has been optimized.

Applications

Human SCF can be used for a variety of applications, including:

Stimulation of proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures.
In vitro expansion of CD34⁺ hematopoietic progenitor cells.
Differentiation of ES-derived cells towards the hematopoietic lineage.
Mast cell differentiation and maintenance.
Mast cell chemotaxis assays.

Alternative names

c-kit ligand, steel factor, MGF

Detailed product information

Background information

SCF is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule.

Biological activity

Proliferation of TF-1 cells (NIBSC 91/682)
research grade: ≥ 2×
10
⁵
U/mg
premium grade: ≥ 4×
10
⁵
U/mg

(Typical specific activity: ≥ 9×
10
⁵
U/mg
)
We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human SCF

Documents and Protocols

Background information

Premium-grade cytokine benefits

App notes/customer reports

How to switch to optimal activity-based cytokine dosing

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for Human SCF

Publications

Lamsfus-Calle, A. et al. (2020) Comparative targeting analysis of KLF1, BCL11A, and HBG1/2 in CD34 + HSPCs by CRISPR/Cas9 for the induction of fetal hemoglobin. Sci Rep 10(1): 10133
Napolitano, R. et al. (2020) Kevetrin induces apoptosis in TP53 wild‑type and mutant acute myeloid leukemia cells. Oncol Rep. 44(4): 1561-1573
Cypris, O. et al. (2019) Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells. Clin Epigenetics 11(1): 19
Xie, Z. S. et al. (2019) Sphingolipid Modulation Activates Proteostasis Programs to Govern Human Hematopoietic Stem Cell Self-Renewal. Cell Stem Cell 25(5): 639-653
Li, J. et al. (2017) Aryl hydrocarbon receptor activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs human B lymphopoiesis. Toxicology 378: 17-24
Arbesu, I. et al. (2016) Platelet-borne complement proteins and their role in platelet-bacteria interactions. J. Thromb. Haemost. 14(11): 2241-2252
Nakazawa, Y. et al. (2016) Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34(+) cells of juvenile myelomonocytic leukemia. J Hematol Oncol. 9: 27
Zhang, J. et al. (2016) Deregulation of DUX4 and ERG in acute lymphoblastic leukemia. Nat. Genet. 48(12): 1481-1489
Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget. 6: 18956-18965
Huan, J. et al. (2015) Coordinate regulation of residual bone marrow function by paracrine trafficking of AML exosomes. Leukemia 29(12): 2285-2295
Krönke, J. et al. (2015) Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS. Nature 523(7559): 183-188
Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
Brault, J. et al. (2014)
Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
ex vivo
models of X
⁰
-linked, AR22
⁰
- and AR47
⁰
- chronic granulomatous diseases.
Biores Open Access 3(6): 311-326
Dighe, N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
Kitumura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334

Data and images