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Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide. |
A: | B: |
Unseparated fraction | Dead cell-depleted fraction |
Dead Cell Removal KitFigure 1Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide. | Dead Cell Removal KitFigure 1Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide. |
We prepare single cells from various tissues for our Genomic studies. We encountered lot of damaged and dead cells in the end from the preparation. This kit works works on MACS principle, fairly simple and easy to use. We use this kit to remove dead cells from the cells suspension as part of our procedure.
Our lab studies ways to enhance immune responses against HIV infection. As such we perform a lot of in vitro killing assays with different effector cells against HIV infected PBMC. One way to measure the outcome of these assays is to look at the number of dead cells after incubating the effectors and targets together in culture. It is very important to start with healthy cells in the beginning of the assay to ensure trusted results in the end. We use the dead cell removal kit for these types of experiments.
Our lab studies HIV immunology and the enrichment of live cells for downstream analysis is important for many of our protocols. This kit provides a reliable way to remove dead cells while maintaining a highly enriched population of live cells for downstream applications.
Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide. |
A: | B: |
Unseparated fraction | Dead cell-depleted fraction |
Dead Cell Removal KitFigure 1Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide. | Dead Cell Removal KitFigure 1Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide. |
We prepare single cells from various tissues for our Genomic studies. We encountered lot of damaged and dead cells in the end from the preparation. This kit works works on MACS principle, fairly simple and easy to use. We use this kit to remove dead cells from the cells suspension as part of our procedure.
Our lab studies ways to enhance immune responses against HIV infection. As such we perform a lot of in vitro killing assays with different effector cells against HIV infected PBMC. One way to measure the outcome of these assays is to look at the number of dead cells after incubating the effectors and targets together in culture. It is very important to start with healthy cells in the beginning of the assay to ensure trusted results in the end. We use the dead cell removal kit for these types of experiments.
Our lab studies HIV immunology and the enrichment of live cells for downstream analysis is important for many of our protocols. This kit provides a reliable way to remove dead cells while maintaining a highly enriched population of live cells for downstream applications.
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