Clone:
CK3-6H5
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
ICFC, MICS, IF, IHC
Alternative names:
KRT7, KRT8, KRT18, KRT19

Extended validation for Cytokeratin Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Cytokeratin Antibody, anti-human

Overview

Cytokeratins are typical intermediate filaments of the cytoskeleton of epithelial cells. They are a biochemically highly diverse multigene family of polypeptides with molecular masses ranging from 40 to 68 kDa. Until now 20 distinct cytokeratin polypeptides have been described, which can be divided into an acidic (type I) and a neutral-basic (type II) subfamily. The cytokeratin expression profile within one cell characterizes the type of epithelia and also the degree of maturation or differentiation. Most malignant cells which have their origin in epithelial tissue express a certain cytokeratin profile, which can be used for classifying carcinomas and for distinguishing carcinomas from malignant tumors of nonepithelial origin. The Anti-Cytokeratin (CK3-6H5) antibody is a pan-cytokeratin–specific antibody targeting cytokeratins from simple epithelia. It crossblocks Cam5.2, an antibody specific for cytokeratin 7 and 8, and has been shown to react with native cytokeratin preparations containing cytokeratins 8, 18, and 19 as well as with recombinant cytokeratin 8.

Alternative names

KRT7, KRT8, KRT18, KRT19

Detailed product information

Technical specifications

CloneCK3-6H5
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCytokeratin
Alternative names of antigenKRT7, KRT8, KRT18, KRT19
Molecular mass of antigen [kDa]44-54
Entrez Gene ID3855, 3856, 3875, 3880
RRIDAB_2784333, AB_244190, AB_2784334

Resources for Cytokeratin Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Cytokeratin Antibody, anti-human

Publications

  1. Makin, C. A. et al. (1984) Monoclonal antibody to cytokeratin for use in routine histopathology. J. Clin. Pathol. 37: 975-983
  2. Balasubramanian, P. et al. (2012) Multiparameter analysis, including EMT markers, on negatively enriched blood samples from patients with squamous cell carcinoma of the head and neck. PLoS One 7(7)
  3. Xin, H. et al. (2014) Establishment and characterization of 7 novel hepatocellular carcinoma cell lines from patient-derived tumor xenografts. PLoS One 9(1): e85308

Related products for
Cytokeratin Antibody, anti-human

4 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?