Clone:
CK3-3E4
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
IHC, WB, ICC, ICFC
Alternative names:
KRT7, KRT8, KRT18, KRT19

Data and images for Cytokeratin Antibody, anti-human

Figures

Figure 1

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Paraffin-embedded tissue sections of an adenocarcinoma of the stomach (intestinal type) were stained with Anti-Cytokeratin (CK3-3E4) pure. The detection was performed using an anti-isotype antibody and a biotin/avidin–horseradish peroxidase system.

Figure 1

Paraffin-embedded tissue sections of an adenocarcinoma of the stomach (intestinal type) were stained with Anti-Cytokeratin (CK3-3E4) pure. The detection was performed using an anti-isotype antibody and a biotin/avidin–horseradish peroxidase system.

Extended validation for Cytokeratin Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
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Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Cytokeratin Antibody, anti-human

Overview

The Anti-Cytokeratin (CK3-3E4) antibody specifically recognizes cytokeratin 8, 18, and 19. It almost completely crossblocks A45-B/B3, an antibody known to recognize the cytokeratin heterodimers 8/18 and 8/19.
The antibody is suitable for immunohistochemistry and Western blotting.

Alternative names

KRT7, KRT8, KRT18, KRT19

Detailed product information

Technical specifications

CloneCK3-3E4
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCytokeratin
Alternative names of antigenKRT7, KRT8, KRT18, KRT19
Molecular mass of antigen [kDa]44-53
Entrez Gene ID3856, 3875, 3880
RRIDAB_244189

Resources for Cytokeratin Antibody, anti-human

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Cytokeratin Antibody, anti-human

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