CD8a
⁺
T Cell Isolation Kit
, mouse

Name: CD8a+ T Cell Isolation Kit, mouse
Availability: InStock

CD8a
⁺
T Cell Isolation Kit
, mouse

The CD8a

⁺

T Cell Isolation Kit, mouse was developed for the untouched isolation of CD8a

⁺

T cells from single-cell suspensions from mouse spleen or lymph node. Purity has been improved with the release of this kit due to the addition of TCRγ/δ antibody to the depletion cocktail.

This kit uses the new fast mouse protocol and replaces the CD8a

⁺

T Cell Isolation Kit II (# 130-095-236).

Product data and images for CD8a
⁺
T Cell Isolation Kit
, mouse

Figures

Figure 1

A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS™ Dissociator. CD8a

⁺

T cells were isolated from this single-cell suspension using the CD8a+ T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD8a T Cell Cocktail (# 130-092-915) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction:

Isolated CD8⁺ T cells:

Figure 1

A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS™ Dissociator. CD8a

⁺

T cells were isolated from this single-cell suspension using the CD8a+ T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD8a T Cell Cocktail (# 130-092-915) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS™ Dissociator. CD8a

⁺

T cells were isolated from this single-cell suspension using the CD8a+ T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD8a T Cell Cocktail (# 130-092-915) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD8a
⁺
T Cell Isolation Kit
, mouse

Overview

The CD8a

⁺

T Cell Isolation Kit, mouse was developed for the untouched isolation of CD8a

⁺

T cells from single-cell suspensions from mouse spleen or lymph node. Purity has been improved with the release of this kit due to the addition of TCRγ/δ antibody to the depletion cocktail.

This kit uses the new fast mouse protocol and replaces the CD8a

⁺

T Cell Isolation Kit II (# 130-095-236).

Detailed product information

Background information

The kit is based on a cocktail of biotin-conjugated antibodies against CD4, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, Ter-119 and TCRγ/δ. The isolation of T cells is achieved by depletion of magnetically labeled cells.

Downstream applications

Untouched T cells are used for functional studies in which cross-linking of surface molecules should be excluded. Isolated T cells can also be further magnetically separated into T cell subsets.

The CD8a

⁺

T Cell Isolation Kit has been used for the following applications: For adoptive transfer experiments

^1,2

, T cell proliferation assays

^2,3

, Coculture with dendritic cells

²

and Cytokine analysis experiments.

Columns

LS or autoMACS

^®

Columns.

Resources for CD8a
⁺
T Cell Isolation Kit
, mouse

Documents and Protocols

Brochures/posters

T cell research - The complete product portfolio

Reviews for CD8a
⁺
T Cell Isolation Kit
, mouse

CD8a+ T Cell Isolation Kit for Mouse

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CD8a+ T Cell Isolation Kit, mouse (130-104-075)

Our lab is interested in isolating CD8 T cells after harvesting spleens from mice. After isolation, we wanted to look at the CD8 T cell gene expression under varying conditions of glutamine concentration. The isolation was reliable at times but not clean other times.

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References for CD8a
⁺
T Cell Isolation Kit
, mouse

Publications

Hamilton-Williams, E. E. et al. (2009) Expression of diabetes-associated genes by dendritic cells and CD4 T cells drives the loss of tolerance in nonobese diabetic mice. J Immunol 183: 1533-1541
Grebe, K. M. et al. (2009)
Sympathetic nervous system control of anti-influenza CD8
⁺
T cell responses.
Proc. Natl. Acad. Sci. U.S.A. 106: 5300-5305
Simma, O. et al. (2009) Identification of an indispensable role for tyrosine kinase 2 in CTL-mediated tumor surveillance. Cancer Res. 69: 203-211

Data and images