Clone:
REA237
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC

Extended validation for CD68 Antibody, anti-rat, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA237
ED1+
Cells were incubated with an excess of purified unconjugated CD68 (REA237) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD68. Wistar rat splenocytes were stained with CD68antibodies and plotted against the side scatter. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant  Analyzer®. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Wistar rat splenocytes were stained with CD68antibodies and plotted against the side scatter. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant  Analyzer®. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Wistar rat splenocytes were stained with CD68antibodies and plotted against the side scatter. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant  Analyzer®. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD68. Wistar rat splenocytes were stained with CD68antibodies and plotted against the side scatter. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant  Analyzer®. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD68 Antibody, anti-rat, REAfinity™

Overview

Clone REA237 recognizes rat CD68, also known as macrosialin, which is a heavily glycosylated type I transmembrane protein of the LAMP (lysosomal-associated membrane protein) family. CD68 is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. It is also present on rat Kupffer cells but absent from rat liver endothelial cells. The expression of rat CD68 is predominantly intracellular, but it is reported that weak cell surface expression also occurs as well. CD68 functions as a macrophage receptor for oxidized low density lipoprotein.
Additional information: Clone REA237 displays negligible binding to Fc receptors.

Detailed product information

Technical specifications

CloneREA237
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesrat
AntigenCD68
Molecular mass of antigen [kDa]35
Distribution of antigenmacrophages, monocytes
Entrez Gene ID287435
RRIDAB_2889619, AB_2659011, AB_2659012, AB_2659015, AB_2659016, AB_2659017, AB_2659018, AB_2889620

Resources for CD68 Antibody, anti-rat, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD68 Antibody, anti-rat, REAfinity™

Publications

  1. Rabinowitz, S. S. et al. (1991) Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli. J. Exp. Med. 174(4): 827-836
  2. Van Velzen, A. G. et al. (1997) Characterization of a receptor for oxidized low-density lipoproteins on rat Kupffer cells: similarity to macrosialin. Biochem. J. 1(322): 411-415

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