CD45RA MicroBeads, human

Name: CD45RA MicroBeads, human
Availability: InStock

CD45RA MicroBeads, human

CD45RA MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.

Product data and images for CD45RA MicroBeads, human

Figures

Figure 1

CD8

⁺

cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8

⁺

CD45RA

⁺

T cells were isolated by positive selection.

Pre-enriched CD8 ⁺ cells	CD8 ⁺ CD45RA ⁺ cells
View details CD45RA MicroBeads, human Figure 1 CD8 ⁺ cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8 ⁺ CD45RA ⁺ T cells were isolated by positive selection.	View details CD45RA MicroBeads, human Figure 1 CD8 ⁺ cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8 ⁺ CD45RA ⁺ T cells were isolated by positive selection.

Specifications for CD45RA MicroBeads, human

Overview

CD45RA MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.

Detailed product information

Background information

CD45RA is expressed on naive CD4

⁺

and CD8

⁺

T cells as well as on CD8

⁺

effector T cells. CD45RA is also present on subsets of B and NK cells as well as on plasmacytoid dendritic cells. The CD45RA antibody recognizes the 220 kDa isoform of the leukocyte common antigen (LCA).

CD45RA MicroBeads are used for the positive selection or depletion of CD45RA

⁺

cells. For isolation of CD45RA

⁺

T cell subsets, the CD45RA MicroBeads can be combined with MACS

^®

Cell Isolation Kits

^6,7

or CD4 and CD8 MultiSort Kits

^1,2

. The CD45RA MicroBeads can also be used to isolate CD45RO

⁺

cells from pre-sorted CD4

⁺

or CD8

⁺

T cells by depletion of CD45RA

⁺

cells.

^3,4,5,7

Downstream applications

Cells isolated with CD45RA MicroBeads can be used for various studies, such as cytokine expression or receptor signaling.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD45RA MicroBeads, human

Documents and Protocols

App notes/customer reports

Effector memory T helper cells secrete IFN-γ upon stimulation with cytokines: a role in chronic inflammation.

References for CD45RA MicroBeads, human

Publications

Wolthers, K. C. et al. (1999) Normal T-cell telomerase activity and upregulation in human immunodeficiency virus-1 infection. Blood 93: 1011-1019
Jonuleit, H. et al. (2000)
Induction of interleukin 10-producing, nonproliferating CD4
⁺
T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells.
J. Exp. Med. 192: 1213-1222
Mattila, P. S. et al. (2000)
Adenoids provide a microenvironment for the generation of CD4
⁺
, CD45RO
⁺
, L-selectin
^–
, CXCR4
⁺
, CCR5
⁺
T lymphocytes, a lymphocyte phenotype found in the middle ear effusion.
Int. Immunol. 12: 1235-1243
Matsushima, T. et al. (2001) Receptor binding cancer antigen expressed on SiSo cells, a novel regulator of apoptosis of erythroid progenitor cells. Blood 98: 313-321
Akdis, M. et al. (1999)
Skin homing (cutaneous lymphocyte-associated antigen-positive) CD8
⁺
T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis.
J Immunol 163: 466-475
Hafsi, N. et al. (2004)
Human dendritic cells respond to
Helicobacter pylori
, promoting NK cell and Tʜ1-effector responses
in vitro
.
J Immunol 173: 1249-1257
Verhasselt, V. et al. (2004) Induction of FOXP3-expressing regulatory CD4pos T cells by human mature autologous dendritic cells. Eur. J. Immunol. 34: 762-772

Data and images

Data and images for CD45RA MicroBeads, human

Figures

Figure 1

CD8

⁺

cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8

⁺

CD45RA

⁺

T cells were isolated by positive selection.

Pre-enriched CD8 ⁺ cells	CD8 ⁺ CD45RA ⁺ cells
View details CD45RA MicroBeads, human Figure 1 CD8 ⁺ cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8 ⁺ CD45RA ⁺ T cells were isolated by positive selection.	View details CD45RA MicroBeads, human Figure 1 CD8 ⁺ cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8 ⁺ CD45RA ⁺ T cells were isolated by positive selection.

Specifications

Specifications for CD45RA MicroBeads, human

Overview

CD45RA MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.

Detailed product information

Background information

CD45RA is expressed on naive CD4

⁺

and CD8

⁺

T cells as well as on CD8

⁺

effector T cells. CD45RA is also present on subsets of B and NK cells as well as on plasmacytoid dendritic cells. The CD45RA antibody recognizes the 220 kDa isoform of the leukocyte common antigen (LCA).

CD45RA MicroBeads are used for the positive selection or depletion of CD45RA

⁺

cells. For isolation of CD45RA

⁺

T cell subsets, the CD45RA MicroBeads can be combined with MACS

^®

Cell Isolation Kits

^6,7

or CD4 and CD8 MultiSort Kits

^1,2

. The CD45RA MicroBeads can also be used to isolate CD45RO

⁺

cells from pre-sorted CD4

⁺

or CD8

⁺

T cells by depletion of CD45RA

⁺

cells.

^3,4,5,7

Downstream applications

Cells isolated with CD45RA MicroBeads can be used for various studies, such as cytokine expression or receptor signaling.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Resources

Resources for CD45RA MicroBeads, human

Documents and Protocols

App notes/customer reports

Effector memory T helper cells secrete IFN-γ upon stimulation with cytokines: a role in chronic inflammation.

References

References for CD45RA MicroBeads, human

Publications

Wolthers, K. C. et al. (1999) Normal T-cell telomerase activity and upregulation in human immunodeficiency virus-1 infection. Blood 93: 1011-1019
Jonuleit, H. et al. (2000)
Induction of interleukin 10-producing, nonproliferating CD4
⁺
T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells.
J. Exp. Med. 192: 1213-1222
Mattila, P. S. et al. (2000)
Adenoids provide a microenvironment for the generation of CD4
⁺
, CD45RO
⁺
, L-selectin
^–
, CXCR4
⁺
, CCR5
⁺
T lymphocytes, a lymphocyte phenotype found in the middle ear effusion.
Int. Immunol. 12: 1235-1243
Matsushima, T. et al. (2001) Receptor binding cancer antigen expressed on SiSo cells, a novel regulator of apoptosis of erythroid progenitor cells. Blood 98: 313-321
Akdis, M. et al. (1999)
Skin homing (cutaneous lymphocyte-associated antigen-positive) CD8
⁺
T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis.
J Immunol 163: 466-475
Hafsi, N. et al. (2004)
Human dendritic cells respond to
Helicobacter pylori
, promoting NK cell and Tʜ1-effector responses
in vitro
.
J Immunol 173: 1249-1257
Verhasselt, V. et al. (2004) Induction of FOXP3-expressing regulatory CD4pos T cells by human mature autologous dendritic cells. Eur. J. Immunol. 34: 762-772

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CD45RA MicroBeads, human

CD45RA MicroBeads, human

Product data and images for CD45RA MicroBeads, human

Figures

Figure 1