Clone:
REA784
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, 3D-IF
Alternative names:
PECAM1, Pecam, EndoCAM, GPIIa

Extended validation for CD31 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA784
390++
MEC13.3-
693102-
REAL260++
Cells were incubated with an excess of purified unconjugated CD31 (REA784) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD31. Splenocytes from BALB/c mice were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Splenocytes from BALB/c mice were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Splenocytes from BALB/c mice were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Splenocytes from BALB/c mice were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Splenocytes from BALB/c mice were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD31. Splenocytes from BALB/c mice were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD31 (REA784). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD31 (REA784). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD31 (REA784). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD31 Antibody, anti-mouse, REAfinity™

Overview

Clone REA784 recognizes the cell surface protein CD31. The murine CD31 antigen is also known as PECAM-1. The encoded protein is a single-pass type I membrane protein containing six Ig-like C2-type (immunoglobulin-like) domains and functions as cell adhesion molecule. CD31 is present on mature endothelial cells as well as on most leukocyte subtypes and platelets where the expression level can vary. Besides its function in exhibiting adhesive properties, the protein is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions.
Additional information: Clone REA784 displays negligible binding to Fc receptors.

Alternative names

PECAM1, Pecam, EndoCAM, GPIIa

Detailed product information

Technical specifications

CloneREA784
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD31
Alternative names of antigenPECAM1, Pecam, EndoCAM, GPIIa
Molecular mass of antigen [kDa]79
Distribution of antigenT cells, NK cells, fibroblasts, granulocytes, endothelial cells, osteoblasts, macrophages, monocytes, lymphocytes, neutrophils
Entrez Gene ID18613
RRIDAB_2657297, AB_2657298, AB_2657299, AB_2657300, AB_2657301, AB_2657302, AB_2657303, AB_2657304, AB_2657305, AB_2657296

References for CD31 Antibody, anti-mouse, REAfinity™

Publications

  1. Xie, Y. and Muller, W. A. (1993) Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1. Proc. Natl. Acad. Sci. U.S.A. 90(12): 5569-5573
  2. Woodfin, A. et al. (2007) PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler. Thromb. Vasc. Biol. 27(12): 2514-2523
  3. van Buul, J. D. and Hordijk, P. L. (2008) Endothelial signaling by Ig-like cell adhesion molecules. Transfus. Clin. Biol. 15(1-2): 3-6

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