CD27 MicroBeads were developed for the positive selection or depletion of CD27
+
cells from human PBMCs or lymphoid tissues. CD27 is differentially expressed on B cells, T cells, and NK cells.

Data and images for CD27 MicroBeads, human

Figures

Figure 1

Positive selection of CD19
+
CD27
+
memory B cells from human PBMCs. CD19
+
B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.
Pre-selected CD19
+
cells
CD19
+
CD27
+
cells
View details

Figure 1

Positive selection of CD19
+
CD27
+
memory B cells from human PBMCs. CD19
+
B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.
View details

Figure 1

Positive selection of CD19
+
CD27
+
memory B cells from human PBMCs. CD19
+
B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.

Specifications for CD27 MicroBeads, human

Overview

CD27 MicroBeads were developed for the positive selection or depletion of CD27
+
cells from human PBMCs or lymphoid tissues. CD27 is differentially expressed on B cells, T cells, and NK cells.

Detailed product information

Background information

CD27 is a member of the tumor necrosis factor receptor family, which among peripheral blood B cells is present on memory B cells
1
and plasma cells but not on naive B cells. In human tonsils, expression of the CD27 antigen is found on memory B cells, germinal center B cells, subepithelial marginal zone B cells, and plasma cells. CD27 is further expressed on specific T cell subsets and NK cell subsets.

Applications

T cells can be pre-selected using the CD8
+
T Cell Isolation Kit or the CD4
+
T Cell Isolation Kit and subsequently separated according to CD27 expression.
For the isolation of human memory B cells, we recommend the Memory B Cell Isolation Kit.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for CD27 MicroBeads, human

Publications

  1. van Lier, R. A. et al. (1987) Tissue distribution and biochemical and functional properties of Tp55 (CD27), a novel T cell differentiation antigen. J. Immunol. 139: 1589-1596
  2. Bigler, R. D. et al. (1988) S152 (CD27). A modulating disulfide-linked T cell activation antigen. J. Immunol. 139: 21-28
  3. Klein, U. et al. (1998)
    Human immunoglobulin (Ig)M
    +
    IgD
    +
    peripheral blood B cells expressing the CD27 cell surface antigen carry somatically mutated variable region genes: CD27 as a general marker for somatically mutated (memory) B cells.
    J. Exp. Med. 188: 1679-1689
  4. Tangye, S. G. et al. (1998) Identification of functional human splenic memory B cells by expression of CD148 and CD27. J. Exp. Med. 188: 1691-1703
  5. Hintzen, R. Q. et al. (1993) Regulation of CD27 expression on subsets of mature T-lymphocytes. J. Immunol. 151: 2426-2435
  6. Elson, L. H. et al. (1995)
    Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4
    +
    CD27
    lymphocyte subpopulation.
    J. Immunol. 154: 4294-4301
  7. Hintzen, R. Q. et al. (1994) Characterization of the human CD27 ligand, a novel member of the TNF gene family. J. Immunol. 152: 1762-1773
  8. Hamann, D. et al. (1997)
    Phenotypic and functional separation of memory and effector human CD8
    +
    T cells.
    J. Exp. Med. 186: 1407-1418
  9. De Milito, A. et al. (2004) Mechanisms of hypergammaglobulinemia and impaired antigen-specific humoral immunity in HIV-1 infection. Blood 103: 2180-2186

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