CD24 Antibody, anti-mouse
Clone:
M1/69
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
CD24A, HSA, Ly-52, Nectadrin

Extended validation for CD24 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with M1/69
REA743+
30-F1++
Cells were incubated with an excess of purified unconjugated CD24 (M1/69) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD24 Antibody, anti-mouse

Overview

The monoclonal antibody M1/69 recognizes mouse CD24, a glycosyl phosphatidylinositol -anchored cell adhesion molecule also known as HSA (heat-stable antigen), which is a marker of hematopoietic differentiation in B and T cell lineages. It is also expressed by thymocytes, erythrocytes and myeloid lineage cells. By controlling the proliferation and differentiation balance between transit-amplifying and committed differentiated cells, CD24 regulates cell differentiation.

Alternative names

CD24A, HSA, Ly-52, Nectadrin

Detailed product information

Technical specifications

CloneM1/69
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD24
Alternative names of antigenCD24A, HSA, Ly-52, Nectadrin
Molecular mass of antigen [kDa]3
Distribution of antigenT cells, B cells, epithelial cells, granulocytes, Langerhans cells, monocytes, red blood cells, leukemia cells, transitional B cells
Entrez Gene ID12484
RRIDAB_2656573, AB_2656574, AB_2656575, AB_2656576, AB_2656577, AB_2656578, AB_2656579, AB_2656580, AB_2656581, AB_2656582, AB_2656583, AB_2656584, AB_2656585, AB_2656572

Resources for CD24 Antibody, anti-mouse

Certificates

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to search for Certificates of Analysis (CoA) by lot number.

References for CD24 Antibody, anti-mouse

Publications

  1. Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
  2. Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037
  3. Alterman, L. A. et al. (1990) Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies. Eur. J. Immunol. 20(7): 1597-1602
  4. Gao, M. Q. et al. (2010)
    CD24
    +
    cells from hierarchically organized ovarian cancer are enriched in cancer stem cells.
    Oncogene 29: 2672-2680
  5. Nieoullon, V. et al. (2007) Mouse CD24 is required for homeostatic cell renewal. Cell Tissue Res. 329(3): 457-467

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