CD22 MicroBeads were developed for the positive selection or depletion of mouse B cells from peripheral blood and various tissues such as bone marrow, lymph nodes, and spleen.

Data and images for CD22 MicroBeads, mouse

Figures

Figure 1

Separation of CD22
+
B cells from spleen suspension using CD22 MicroBeads, an MS Column, and a MidiMACS
Separator. Cells were fluorescently stained and analyzed by flow cytometry using MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction:
CD22+ cells:
CD22- cells:
View details

Figure 1

Separation of CD22
+
B cells from spleen suspension using CD22 MicroBeads, an MS Column, and a MidiMACS
Separator. Cells were fluorescently stained and analyzed by flow cytometry using MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of CD22
+
B cells from spleen suspension using CD22 MicroBeads, an MS Column, and a MidiMACS
Separator. Cells were fluorescently stained and analyzed by flow cytometry using MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of CD22
+
B cells from spleen suspension using CD22 MicroBeads, an MS Column, and a MidiMACS
Separator. Cells were fluorescently stained and analyzed by flow cytometry using MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD22 MicroBeads, mouse

Overview

CD22 MicroBeads were developed for the positive selection or depletion of mouse B cells from peripheral blood and various tissues such as bone marrow, lymph nodes, and spleen.

Detailed product information

Background information

CD22 is expressed on B lineage cells throughout their development from the early pro-B cell stages onwards and is down-regulated upon terminal differentiation to plasma cells.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

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