CD208 (DC-LAMP) Antibody, anti-human, REAfinity™

Name: CD208 (DC-LAMP) Antibody, anti-human, REAfinity™
Availability: InStock

CD208 (DC-LAMP) Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA295

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

ICFC

Alternative names:

LAMP3, DC LAMP, Protein TSC403

Intracellular flow cytometry applications forCD208 (DC-LAMP) Antibody, anti-human, REAfinity™

APC
PE

Conjugate:

APC

Recommended dilution:

1:50

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-122-229, 130-122-220

Protocols:

Intracellular flow cytometry staining protocol (Inside Stain Kit 1:50)

Description:

Mature human monocyte-derived dendritic cells were fixed and permeabilized. Cells were then stained with CD208 (DC-LAMP) antibodies or with the corresponding REA Control (I) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant^® Analyzer. CD83⁺ cells were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.

Extended validation for CD208 (DC-LAMP) Antibody, anti-human, REAfinity™

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with REA295
I10-1112	+
IMD31B	-
104.G4	-
Cells were incubated with an excess of purified unconjugated CD208 (DC-LAMP) (REA295) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD208 (DC-LAMP). Mature MoDc cells were stained with CD208 (DC-LAMP) antibodies and with a suitable counterstaining. As a control, CD208 (DC-LAMP) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD208 (DC-LAMP). Mature MoDc cells were stained with CD208 (DC-LAMP) antibodies and with a suitable counterstaining. As a control, CD208 (DC-LAMP) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD208 (DC-LAMP). Mature MoDc cells were stained with CD208 (DC-LAMP) antibodies and with a suitable counterstaining. As a control, CD208 (DC-LAMP) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD208 (DC-LAMP) Antibody, anti-human, REAfinity™

Overview

Clone REA295 recognizes the CD208 antigen, a single-pass type I intracellular protein also known as lysosome-associated membrane glycoprotein 3 (LAMP-3) or DC-lysosome–associated membrane glycoprotein (DC-LAMP). CD208 was first cloned as a gene specifically expressed in lung tissues and found to be overexpressed in primary cancers of the esophagus, colon, fallopian tube, ovary, breast, and liver, although its expression was barely detectable in the corresponding normal tissues. It is almost exclusively expressed by mature dendritic cells (DCs). Expression can be induced in CD34

⁺

derived dendritic cells by stimulation with GM-CSF and TNF-α or in monocytes by the stimulation with GM-CSF and IL-4. CD208 is found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes. This suggests that CD208 might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of DC.

Additional information: Clone REA295 displays negligible binding to Fc receptors.

Alternative names

LAMP3, DC LAMP, Protein TSC403

Detailed product information

Technical specifications

Clone	REA295
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	CD208 (DC-LAMP)
Alternative names of antigen	LAMP3, DC LAMP, Protein TSC403
Molecular mass of antigen [kDa]	42
Distribution of antigen	dendritic cells
Entrez Gene ID	27074
RRID	AB_2811380, AB_2656242, AB_2656243, AB_2811382

Resources for CD208 (DC-LAMP) Antibody, anti-human, REAfinity™

Certificates

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References for CD208 (DC-LAMP) Antibody, anti-human, REAfinity™

Publications

de Saint-Vis, B. et al. (1998) A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. Immunity 9(3): 325-336
Bell, D. et al. (1999) In breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas. J. Exp. Med. 190(10): 1417-1426
Kanao, H. et al. (2005) Overexpression of LAMP3/TSC403/DC-LAMP promotes metastasis in uterine cervical cancer. Cancer Res. 65(19): 8640-8645

Applications

Extended validation