Clone:
REAL518
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
MMR

Specifications for CD206 Antibody, anti-human, REAdye_lease™

Overview

Clone REAL518 is an antibody fragment derived from the full CD206 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL518 recognizes the human CD206 antigen, also known as Mannose receptor (MR) or macrophage MR, which is a 162–175 kDa type I membrane protein. It contains an N-terminal cystein-rich domain and eight C-type lectin-like domains (CTLDs), beside a transmembrane and a cytoplasmic domain. CD206 is expressed by macrophages, dendritic cells, and subsets of endothelial cells, but not on monocytes. The MR recognizes multiple mainly microbial carbohydrates with mannose, fucose, or N-acetyl glucosamine residues. The MR mediates endocytosis and phagocytosis, linked to antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

MMR

Detailed product information

Technical specifications

CloneREAL518
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD206
Alternative names of antigenMMR
Distribution of antigendendritic cells, macrophages, endothelial cells, lymph node
RRIDAB_2857557, AB_2857559

References for CD206 Antibody, anti-human, REAdye_lease™

Publications

  1. Tacken, P. J. et al. (2005) Effective induction of naive and recall T-cell responses by targeting antigen to human dendritic cells via a humanized anti-DC-SIGN antibody. Blood 106: 1278-1285
  2. Ezekowitz, R. A. B. et al. (1990) Molecular characterization of the human macrophage mannose receptor: demonstration of multiple carbohydrate recognition-like domains and phagocytosis of yeasts in Cos-1 cells. J. Exp. Med. 172: 1785-1794
  3. Kang, P. B. et al. (2005) The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabinomannan-mediated phagosome biogenesis. J. Exp. Med. 202: 987-999
  4. Sallusto, F. et al. (1995) Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartement: downregulation by cytokines and bacterial products.​ J. Exp. Med. 182: 389-400
  5. Wileman, T. E. et al. (1986) Identification of the macrophage mannose receptor as a 175-kDa membrane protein. Proc. Natl. Acad. Sci. U.S.A. 83: 2501-2505
  6. Feinberg, H. et al. (2000) Structure of a C-type carbohydrate recognition domain from the macrophage mannose receptor. J. Biol. Chem. 275: 21539-21548
  7. Lennartz, M. R. et al. (1989) Biosynthesis and processing of the mannose receptor in human macrophages. J. Biol. Chem. 264: 2385-2390
  8. Yee Seng Ng (2012) Chemical transfection of dye-conjugated microRNA precursors for microRNA functional analysis of M2 macrophages. J. Cell. Biochem. 113(5): 1714-1723