CD1a MicroBeads, human

CD1a MicroBeads have been developed for positive selection of CD1a
+
dendritic cells from epidermis (Langerhans cells), dermis, lung, mucosa, and other non-lymphoid tissues or for further purification of CD1a
+
dendritic cells generated
ex vivo
from CD34
+
or CD133
+
hematopoietic progenitor cells.

Data and images for CD1a MicroBeads, human

Figures

Figure 1

View details
Dendritic cells were generated by culturing isolated CD133
+
cells in the presence of Flt-3 Ligand, SCF, GM-CSF, TNF-α, and TGF-β for 10 days. Thereafter, CD1a
+
cells were isolated by using CD1a MicroBeads and stained with CD1a-FITC.

Figure 1

Dendritic cells were generated by culturing isolated CD133
+
cells in the presence of Flt-3 Ligand, SCF, GM-CSF, TNF-α, and TGF-β for 10 days. Thereafter, CD1a
+
cells were isolated by using CD1a MicroBeads and stained with CD1a-FITC.

Separation of human CD1a
+
dendritic cells

View details
CD133
+
hematopoietic progenitor cells were isolated using the CD133 MicroBead Kit and were cultured for 12 days in the presence of Flt-3 ligand, TGF-β, TNF-α, GM-CSF, and SCF. Thereafter, CD1a
+
dendritic cells were isolated using the CD1a MicroBeads, an MS Column, and a MiniMACS™ Separator.
Cells were fluorescently stained with CD1a-FITC and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
CD133
+
hematopoietic progenitor cells were isolated using the CD133 MicroBead Kit and were cultured for 12 days in the presence of Flt-3 ligand, TGF-β, TNF-α, GM-CSF, and SCF. Thereafter, CD1a
+
dendritic cells were isolated using the CD1a MicroBeads, an MS Column, and a MiniMACS™ Separator.
Cells were fluorescently stained with CD1a-FITC and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD1a MicroBeads, human

Overview

CD1a MicroBeads have been developed for positive selection of CD1a
+
dendritic cells from epidermis (Langerhans cells), dermis, lung, mucosa, and other non-lymphoid tissues or for further purification of CD1a
+
dendritic cells generated
ex vivo
from CD34
+
or CD133
+
hematopoietic progenitor cells.

Detailed product information

Background information

The CD1a antigen is a member of the CD1 family of proteins, which are structurally related to MHC class I proteins and mediate the presentation of non-peptide antigens to T cells.
1

Applications

CD1a MicroBeads were used, for example, for the isolation of Langerhans cells from epidermis
2
or for isolation of hematopoietic progenitor cell-derived dendritic cells.
3

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. Large Cell Columns are recommended for the isolation of Langerhans cells from skin.

References for CD1a MicroBeads, human

Publications

  1. Brigl, M. and Brenner, M. B. (2004) CD1: antigen presentation and T cell function. Annu. Rev. Immunol. 22: 817-890
  2. Simon, J. C. et al. (1995) Rapid purification of human Langerhans cells using paramagnetic microbeads. Exp. Dermatol. 4: 155-161
  3. Ye, Z. et al. (1996)
    In vitro
    expansion and characterization of dendritic cells derived from human bone marrow CD34
    +
    cells.
    Bone Marrow Transplant. 18: 997-1008

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