CD158f (KIR2DL5) Antibody, anti-human

Name: CD158f (KIR2DL5) Antibody, anti-human
Availability: InStock

CD158f (KIR2DL5) Antibody, anti-humanExtended Validation

Clone:

UP-R1

Type of antibody:

Primary antibodies

Isotype:

mouse IgG1κ

Applications:

FC

Alternative names:

KIR2DL5A, KIR2DL5B

Flow cytometry applications forCD158f (KIR2DL5) Antibody, anti-human

APC
APC-Vio 770
PE
PE-Vio 770
pure

Conjugate:

APC

Recommended dilution:

1:11

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-098-567, 130-098-569

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:11)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with CD158f (KIR2DL5) antibodies or with the corresponding isotype control antibodies (left images) as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Extended validation for CD158f (KIR2DL5) Antibody, anti-human

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD158f (KIR2DL5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158f (KIR2DL5) antibodies and with a suitable counterstaining. As a control, CD158f (KIR2DL5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD158f (KIR2DL5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158f (KIR2DL5) antibodies and with a suitable counterstaining. As a control, CD158f (KIR2DL5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD158f (KIR2DL5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158f (KIR2DL5) antibodies and with a suitable counterstaining. As a control, CD158f (KIR2DL5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD158f (KIR2DL5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158f (KIR2DL5) antibodies and with a suitable counterstaining. As a control, CD158f (KIR2DL5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD158f (KIR2DL5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158f (KIR2DL5) antibodies and with a suitable counterstaining. As a control, CD158f (KIR2DL5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD158f (KIR2DL5) (UP-R1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD158f (KIR2DL5) (UP-R1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD158f (KIR2DL5) Antibody, anti-human

Overview

Clone UP-R1 recognizes CD158f (KIR2DL5), a member of the killer immunoglobulin-like receptor (KIR) family which recognizes subsets of HLA alleles. Expression is found mainly on CD56

^dim

CD16

⁺

natural killer (NK) cells but also on a subset of CD8

⁺

T cells. KIRs are monomeric receptors possessing high allelic polymorphism, with either two or three Ig-like extracellular domains.

Alternative names

KIR2DL5A, KIR2DL5B

Detailed product information

Technical specifications

Clone	UP-R1
Clonality	monoclonal
Isotype	mouse IgG1κ
Isotype control	Isotype Control Antibody, mouse IgG1
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD158f (KIR2DL5)
Alternative names of antigen	KIR2DL5A, KIR2DL5B
Molecular mass of antigen [kDa]	38
Distribution of antigen	NK cells, T cells
Entrez Gene ID	57292
RRID	AB_2733099, AB_2660359, AB_2660360, AB_2660361, AB_2660362, AB_2660363, AB_2660366, AB_2733098

Resources for CD158f (KIR2DL5) Antibody, anti-human

Certificates

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Applications

Extended validation